利用Pseudorabies病毒做透過神經運輸以探討中樞神經至精囊的神經支配
Date Issued
2001
Date
2001
Author(s)
謝汝敦
DOI
892314B002354
Abstract
After exposing the seminal vesicle of the mature male Wistar rats, horseradish
peroxidase (HRP) was injected into the wall of the seminal vesicle in 8-12 separated
areas. The rats were sacrificed 72 hours later. We used phosphate buffered saline (PBS)
for transcardial perfusion and followed by fixatives (1.25% glutaldehyde + 1%
paraformaldehyde). The tissues of spinal segments T10-L2, L5-S3 and the major
pelvic ganglia were removed and fixed in previous fixatives (1.25% glutaldehyde +
1% paraformaldehyde) and immersion in phosphate buffer (30% PBS, 0.1 M, pH 7.4,
4 oC) for another 3 days. The frozen specimens were cut into slices with OCT frozen
medium and followed with tetramethylbenzidine (TMB) staining before HRP
histochemical reaction. After treated with enzymatic reaction solution, the specimens
were dried and stained with 1 % neutral red for 3 minutes and followed with light
microscopic examination.
10 mature male Wistar rats were used in this study. Only 2 of the final six rats
showed faint brownish red staining particles on the major pelvic ganglia. In the
segments of spinal cord from T10 toL2 and L5 to S3, all of them did not show any
evidence of horseradish peroxidase tracing.
Publisher
臺北市：國立臺灣大學醫學院泌尿科
Type
other
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