大白鼠精囊血清素系統之探討

Abstract

Using our established animal model by stimulating the LSN and monitoring the rat seminal vesicle, we evaluated some of the subtypes of serotonergic agonists including 8-OH-DPAT (1A), 5-Nonyloxytryptamine (1B), BW723C86 (2B) and MK 212 (2C). Mature male Wistar rats (12-14 weeks) were used in this study. All of these test drugs from 10-6 to 10-4 M/kg were injected cumulatively via the catheter of femoral artery. The phasic tension (ΔmmHg) of each drug at different concentration will be compared to the control to obtain the dose-response curve of each subtype of 5-HT agonist. From the dose-response curve, only 8-OH-DPAT (5-HT1A) had a dose-dependent inhibition on the seminal vesicle pressure response to the LSN stimulation. More than 50% of inhibition was noted at the concentration of 104 M/kg of 8-OH-DPAT, and the IC50 of 8-OH-DPAT was 6.46 x 10-5 M/kg. We believed that 8-OH-DPAT (5-HT1A) mediates the rat seminal vesicle contraction, not like 5-HT2C receptors in the spinal cord reflex center. However, further investigation, as immuno-histochemical study is necessary to prove it.