Using our established animal model by stimulating the LSN and monitoring the rat
seminal vesicle, we evaluated some of the subtypes of serotonergic agonists including
8-OH-DPAT (1A), 5-Nonyloxytryptamine (1B), BW723C86 (2B) and MK 212 (2C).
Mature male Wistar rats (12-14 weeks) were used in this study. All of these test drugs
from 10-6 to 10-4 M/kg were injected cumulatively via the catheter of femoral artery.
The phasic tension (ΔmmHg) of each drug at different concentration will be
compared to the control to obtain the dose-response curve of each subtype of 5-HT
agonist. From the dose-response curve, only 8-OH-DPAT (5-HT1A) had a
dose-dependent inhibition on the seminal vesicle pressure response to the LSN
stimulation. More than 50% of inhibition was noted at the concentration of 104 M/kg
of 8-OH-DPAT, and the IC50 of 8-OH-DPAT was 6.46 x 10-5 M/kg.
We believed that 8-OH-DPAT (5-HT1A) mediates the rat seminal vesicle
contraction, not like 5-HT2C receptors in the spinal cord reflex center. However,
further investigation, as immuno-histochemical study is necessary to prove it.
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