|Title:||Characterization of Chemoresistance Mechanisms in a Series of Cisplatin- Resistant Transitional Carcinoma Cell Lines
HUANG, CHAO- YUAN
|Keywords:||cisplatin;glutathione;glutathionetransferase;drug resista;multiple;carcinoma, transitional cell||Issue Date:||2000||Journal Volume:||v.20||Journal Issue:||n.5A||Start page/Pages:||3221-3226||Source:||ANTICANCER RESEARCH||Abstract:||
We explored the mechanisms of cisplatin resistance in a series of bladder transitional carcinoma cells that are either sensitive or progressively resistant to cisplatin. Resistant lines were raised by chronic exposure of the parental cells to progressively increased concentrations of cisplatin . The cisplatin IC50s of the sensitive and the three resistant cells were 4.3, 25.0, 40.4, and 52.2 mM, respectively. The expressions of glutathione S-transferase p (GST-p) and multidrug resistance-associated protein (MRP) were enhanced in a dose-response manner as cells acquired progressive cisplatin resistance. Expression of mdr-1 transcript was detected in the three resistant lines but not in the sensitive line. Glutathione contents were increased in resistant cells, yet the trend of increase did not reach statistical significance (p=0.061). In conclusion, transitional carcinoma cells may gain cisplatin resistance through multiple pathways including up-regulation of GST-p, MRP and possibly mdr-1. Glutathione contents
|Appears in Collections:||醫學系|
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