https://scholars.lib.ntu.edu.tw/handle/123456789/193812
DC Field | Value | Language |
---|---|---|
dc.contributor | 泌尿科 | en |
dc.contributor.author | Gray, Margaret A. | en |
dc.contributor.author | Wang, Chung-Cheng | en |
dc.contributor.author | Sacks, Michael S. | en |
dc.contributor.author | Yoshimura, Naoki | en |
dc.contributor.author | Chancellor, Michael B. | en |
dc.contributor.author | Nagatomi, Jiro | en |
dc.creator | 王炯珵 | zh-tw |
dc.creator | Gray, Margaret A.; Wang, Chung-Cheng; Sacks, Michael S.; Yoshimura, Naoki; Chancellor, Michael B.; Nagatomi, Jiro | en |
dc.date | 2008 | en |
dc.date.accessioned | 2009-12-23T08:35:19Z | - |
dc.date.accessioned | 2018-07-11T17:16:23Z | - |
dc.date.available | 2009-12-23T08:35:19Z | - |
dc.date.available | 2018-07-11T17:16:23Z | - |
dc.date.issued | 2008 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/175131 | - |
dc.description.abstract | OBJECTIVES To investigate time-course changes in the expression of select genes and extracellular matrix proteins in the bladder of rats with streptozotocin-induced diabetes , so as to examine the mechanisms underlying changes in mechanical properties of the bladder due to diabetic cystopathy. METHODS Female Sprague-Dawley rats were injected with streptozotocin (65 mg/kg). Rats that were fed with 5% sucrose in drinking water served as diuretic controls, in addition to normal control rats. At the end of 2, 4, and 8 weeks, total ribonucleic acid (RNA) isolated from the detrusor layer of the bladders was reverse transcribed, and then complementary deoxyribonucleic acid was amplified with polymerase chain reaction primer sets for type I Collagen, type III Collagen, tropoelastin, and transforming growth factor beta 1 (TGF-beta-1). Collagen and elastin contents of the bladders were quantified with commercially available assays. RESULTS Both diabetic and diuretic rat bladders exhibited significantly (P <0.05) lower expression of type I Collagen and TGF-beta-1 messenger RNA (mRNA) compared with normal controls at all time points tested. In contrast, downregulation of type III Collagen mRNA expression in both diabetic and diuretic groups was seen at 4 and 8 weeks. Furthermore, tropoelastin mRNA expression in the diabetic rat bladders was , compared with normal and diuretic rats, significantly (P <0.05) greater at 2 weeks. Both diabetic and diuretic rat bladders exhibited significantly (P <0.05) decreased Collagen and increased elastin protein content at 2 and 8 weeks. CONCLUSIONS The results of the present study suggest that increases in compliance of the bladders in diabetic cystopathy result not only from diuresis-driven reduction of Collagen synthesis but also from increased elastin synthesis. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | UROLOGY v.71 n.6 pp.1214-1219 | en |
dc.relation.ispartof | Urology | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | collagen type 1; collagen type 3; complementary DNA; RNA; streptozocin; transforming growth factor beta1; tropoelastin; animal experiment; animal model; article; controlled study; diabetes mellitus; down regulation; female; gene expression; nonhuman; priority journal; protein content; rat; reverse transcription polymerase chain reaction; Animals; Diabetes Mellitus, Experimental; Extracellular Matrix Proteins; Female; Gene Expression; Rats; Rats, Sprague-Dawley; Time Factors; Urinary Bladder | - |
dc.title | Time-Dependent Alterations of Select Genes in Streptozotocin-Induced Diabetic Rat Bladder | en |
dc.identifier.doi | 10.1016/j.urology.2007.11.054 | - |
dc.relation.pages | 1214-1219 | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
Appears in Collections: | 醫學系 |
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