Unilateral Ureteral Obstruction Evokes Renal Tubular Apoptosis Via the Enhanced Oxidative Stress and Endoplasmic Reticulum Stress in the Rat
Resource
NEUROUROLOGY AND URODYNAMICS v.30 n.3 pp.472-479
Journal
Neurourology and Urodynamics
Pages
472-479
Date Issued
2011
Date
2011
Author(s)
YEH, CHUNG-SHINN
CHIANG, HAN-SUN
LAI, TING-YU
CHIEN, CHIANG-TING
Abstract
Purpose: Oxidative stress and endoplasmic reticulum ER stress may induce renal apoptosis and contribute to the pathogenesis of the kidney with unilateral ureteral obstruction UUO. Materials and Methods: We induced UUO the female Wistar rats by ligation of the left ureter at the ureteropelvic junction. TheUUOkidney was performed from 4 hr to 7 days course. At the indicated time, we measured the arterial blood pressure and renal blood flow in each rat, renal ROS measurement in vivo by a chemiluminescence analyzer. We performed immunohistochemistry of monocyte/ macrophage ED-1 stain for leukocyte infiltration, 4- hydroxynoneal 4-HNE stain for ROS products, and apoptosis by terminal deoxynucleotidyl transferase-mediated nick-end labeling TUNEL and Western blot to analyze ER stress- associated andapoptosis-related proteins expression intheUUOkidney. Results: WefoundthatUUOdecreasedrenal bloodflowand increased renal vascular resistance and renal ROS. UUOdecreased renal manganese superoxide dismutaseMnSOD and catalase protein expression in a time-dependent manner. Increased-4-HNEstain in the renal tubules and ED-1 stain in the renal tubulointerstitial compartment occurred after 4 hr of UUO in the kidney. UUO significantly enhanced ER stress markers likeERstress-response protein 25 and glucose- regulated protein 78 and ER- associated apoptosis proteins, c -JUN NH2-terminal kinase, and caspase 12, in the kidney. Subsequently, UUOenhanced renal pro-apoptotic Bax and caspase 3 expression and decreased anti-apoptotic Bcl-2 expression, leading to renal tubular apoptosis. Conclusions: Our data suggest that renal tubular apoptosis induced by oxidative stress and ER stress occurred in the UUO kidney.
Subjects
apoptosis
endoplasmic reticulum stress
kidney
oxidative stress
unilateral ureteral obstruction
SDGs