DC 欄位 | 值 | 語言 |
dc.contributor.author | 黃立民 | zh_TW |
dc.creator | 黃立民 | zh_TW |
dc.date | 2002 | zh_TW |
dc.date.accessioned | 2006-07-26T03:07:37Z | - |
dc.date.accessioned | 2018-07-11T17:50:06Z | - |
dc.date.available | 2006-07-26T03:07:37Z | - |
dc.date.available | 2018-07-11T17:50:06Z | - |
dc.date.issued | 2002 | - |
dc.identifier | 902314B002160 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/22869 | - |
dc.description.abstract | Vpr 為愛滋病毒附屬基因蛋白由96 個
氨基酸所組成。病毒生殖週期中Vpr 執行
的多效性功能包括核換位,細胞週期阻
斷,異位活化,增進病毒複製,與細胞凋
亡。酵母菌雙混種系統是鑑定蛋白與蛋白
作用之利器,本實驗室已成功地利用此系
統尋找到一些會與Vpr 蛋白作用之細胞蛋
白,包括訊息傳遞蛋白HAX-1,脊椎肌肉
萎縮基因蛋白SMN1(survival of motor
neuron 1, human spinal muscular atrophy
disease gene)。進一步的研究發現在HeLa
細胞株同時表達HAX-1 與Vpr-GFP 螢光標
定蛋白時,N 端與全長的HAX-1 蛋白均有阻
斷Vpr 蛋白核換位(nuclear transport)的能
力;而C 端則不具此阻斷能力(blocking
function)。我們假定HAX-1 停滯病毒蛋白於
細胞質之功能(cytoplasmic retention)必
定造成宿主細胞功能的改變繼而影響病毒
感染之致病機轉。關於Vpr 與SMN1 相互作
用的功能意義較難釐清是基於神經蛋白
SMN1 如何誘導運動神經失常
(degeneration of motor neuron)造成肌
肉萎縮(muscular atrophy)仍是待解的謎
題。相關研究指出Vpr 會造成神經細胞的
凋亡(apoptosis),部份研究亦證明SMN1
與抗細胞凋亡的蛋白NAIP(neuronal
apoptosis inhibitory protein)及Bcl-2
都有關係,我們未來的研究將進一步探究
SMN1 與Vpr 之間功能聯結的關係。 | zh_TW |
dc.description.abstract | HIV-1 Vpr, a 96-amino-acid 14-kDa
protein, has several important and interesting
functions, including nuclear translocation of
the pre-integration complex, cell cycle arrest
at the G2/M phase, transactivation,
enhancement of virus replication, and
apoptosis. To understand the role of Vpr in
HIV-1 life cycle and pathogenesis, yeast
two-hybrid system had been used and cDNA
encoding HAX-1 or SMN1 protein was
identified. Interaction between Vpr and
HAX-1 (or SMN1) was characterized by in
vitro protein-protein binding assay.
Furthermore, over-expressed HAX-1 or
N-terminal of HAX-1 in HeLa cells blocks
the nuclear transport of a Vpr-GFP fusion
protein while C-terminal of HAX-1 fail to
block Vpr-GFP nuclear transport. We
hypothesize that cytoplasmic retention of
HIV-1 regulatory protein Vpr by
protein-protein interaction with human
cytoplasmic protein HAX-1 may cause
functional changes in the host cell that affect
HIV-1 pathogenesis.
It is difficult to evaluate the interaction
of Vpr and SMN1 since little is known about
SMN1 how to cause the degeneration of
motor neuron the developing to spinal
muscular atrophy disease. Newly studies find
that Vpr induces apoptosis in neuronal cells,
and the correlations between SMN1 and
antiapoptosis protein, NAIP (neuronal
apoptosis inhibitory protein) and Bcl-2, has
been proved. In coming project we will try to
verify the possible functional mechanism.
This article provides guidance for report writing
under the Grant of National Science Council
beginning from fiscal year 1998. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 276321 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院小兒科 | zh_TW |
dc.rights | 國立臺灣大學醫學院小兒科 | zh_TW |
dc.subject | 愛滋病毒 | zh_TW |
dc.subject | 酵母菌雙混種系統 | zh_TW |
dc.subject | Vpr | zh_TW |
dc.subject | HAX-1 | zh_TW |
dc.subject | HIV-1 | en |
dc.subject | yeast two - hybrid system | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | 愛滋病毒Vpr 蛋白之功能研究及其與HAX-1 和脊椎肌肉萎縮基因之作用(1/3) | zh_TW |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/22869/1/902314B002160.pdf | - |
dc.coverage | 計畫年度:90;起迄日期:2001-08-01/2002-07-31 | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.openairetype | report | - |
item.languageiso639-1 | zh_TW | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
顯示於: | 醫學系
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