https://scholars.lib.ntu.edu.tw/handle/123456789/194437
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 李旺祚 | zh_TW |
dc.creator | 李旺祚 | zh_TW |
dc.date | 2002 | zh_TW |
dc.date.accessioned | 2006-07-26T03:07:44Z | - |
dc.date.accessioned | 2018-07-11T17:50:08Z | - |
dc.date.available | 2006-07-26T03:07:44Z | - |
dc.date.available | 2018-07-11T17:50:08Z | - |
dc.date.issued | 2002 | - |
dc.identifier | 902314B002183 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/22874 | - |
dc.description.abstract | 3-Nitropropionic acid (3-NP), an irreversible inhibitor of succinate dehydrogenase, induced ATP depletion and both necrosis and apoptosis in human NT2-N neurons. Necrosis occurred predominantly during the first 2 days in a dose-dependent manner, whereas apoptosis was observed after 24 hr or later at a low constant rate in 0.1mM as well as 5mM 3-NP. We assayed the intracellular concentration of Ca24- ([Ca2j1) during the first 48 hours in 1mM 3-NP, a period during which 10% of the neurons died by necrosis and 3% by apoptosis. During the first 2 hours in 3-NP, all NT2-N neurons showed [Ca2j1 rise from48¡Ó2 to 140¡Ó l2nM(mean¡ÓSEM¡¦). After 24 and 48 hours in 3-NP, however, [Ca24-J remained above IOOnM in only 17% and 25% of the NT2-N neurons, respectively, suggesting that most neurons were able to correct this early rise in [Ca24-], despite severe ATP depletion, and to survive. Activation of NMDA-GIuR contributed substantially to 3-NP-induced ATP depletion, and subsequent chronic elevation of [Ca24-]1 in the NT2-N neurons. We also demonstrated that blocking endoplasmic reticulum (ER) Ca24- release enhanced the capacity of these human neurons to maintain [Ca21], homeostasis and resist necrosis while subjected to chronic energy deprivation. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 688323 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院小兒科 | zh_TW |
dc.rights | 國立臺灣大學醫學院小兒科 | zh_TW |
dc.subject | 3-nitropropionic acid | en |
dc.subject | mitochondrial permeability transition | en |
dc.subject | caspase | en |
dc.subject | antioxidant | en |
dc.subject | apoptosis | en |
dc.subject | NMDA-GIuR | en |
dc.subject | NMDA 受體 | zh_TW |
dc.subject | 鈣離子 | zh_TW |
dc.subject | 內質網 | zh_TW |
dc.title | 粒線體功能異常對人體神經細胞的影響(2/2) | zh_TW |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/22874/1/902314B002183.pdf | - |
dc.coverage | 計畫年度:90;起迄日期:2001-08-01/2002-07-31 | zh_TW |
item.fulltext | with fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | report | - |
item.languageiso639-1 | zh_TW | - |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.grantfulltext | open | - |
顯示於: | 醫學系 |
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902314B002183.pdf | 672.19 kB | Adobe PDF | 檢視/開啟 |
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