|Title:||GTP Cyclohydrolase I 突變及Splicing之分子結構機轉(3/3)||Authors:||胡務亮||Keywords:||GTPcyclohydro lase I;Alternative splicing;Dominant-negative effect;Regulation||Issue Date:||2004||Publisher:||臺北市：國立臺灣大學醫學院小兒科||Start page/Pages:||-||Source:||Biochemical and Biophysical Research Communications (306),937–942||Abstract:||
GTPcyclohydrolase I (GCH, EC 18.104.22.168) regulates the level of tetrahydrobiopterin and in turn the activities of nitric oxide
synthase and aromatic amino acid hydroxylases. Type II GCH mRNA, an alternatively spliced species abundant in blood cells,
encodes a truncated and nonfunctional protein. When we stimulate peripheral blood mononuclear cells by PHA, the transcription of
full-length GCH mRNA increased, but that of type II mRNA decreased transiently. We further demonstrated that the type II
cDNA exerted a dominant-negative effect on the wild-type cDNA, similar to the effect of some GCH mutants. Therefore, type II
mRNA may regulate GCH and then contribute to the regulation of NO production by BH4-dependent iNOS in mononuclear cells.
Selection of the splicing sites may be coupled with transcriptional activation of the GCH gene.
|Appears in Collections:||醫學系|
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