兒童過敏性紫斑症血管內皮細胞自體抗原之探討(1/2)
Date Issued
2004
Date
2004
Author(s)
楊曜旭
DOI
922314B002223
Abstract
Henoch-Schönlein purpura (HSP) is one of the most common types of vasculitis in
childhood. Histologically, it reveals the change of leukocytoclastic vasculitis, and has
been well regarded as a specific clinicopathological entity by the vascular deposition
of immunoglobulin (Ig) A-dominant immune complexes and elevated serum IgA level.
The pathogenesis of HSP remains undetermined. Clinical observations have found
striking seasonal variations in HSP, with most cases occurring in the winter and
autumn. HSP has also been associated with a history of preceding infections,
especially upper respiratory tract infection. These results raise the possibility of
infection as a direct cause or a potential trigger of this disease. It is speculated that
some microorganisms with specific antigenic structures, which mimic some
components of blood vessel enters the respiratory or gastrointestinal tract and
stimulates the T cells residing in the mucosa. Inflammatory responses are elicited with
the participation of various immunocompetent cells and their humoral counterparts.
The antibodies, and other immune cells against the microorganisms may cross-react
with the specific component of the vessel and lead to the inflammation of vessels.
The aims of this project are to design experiments to determine the pathogenesis of
childhood HSP. In the previous 9 months, we have completed the following works: (1)
we have set up and maintain the culture of human endothelial cells. (2) The serum
samples of 20 HSP children were collected at different stages. (3) By means of
immunofluorescence assay and cell-based ELISA, we have confirmed that IgA
anti-endothelial cell antibodies developed during the acute stage of HSP. (4) The
interactions of antibodies (acute sera of patients) and endothelial cells have been
investigated (Yang YH, et al. Ann Rheum Dis 2004). (5) The membrane
protein/cytoplasmic protein have been separated and collected. In the future, we will
further establish the antibodies library of children with HSP. Then, the autoantibodies
against EC will be determined by the screen of the HUVEC cDNA library or EC
membrane and cytoplasmic proteins. Using the screened antibodies or sera of patients
at the acute stage, the disease-specific antigen will be determined by Western blotting
(SDS-PAGE and 2-D immunoblotting). If this study can be performed and completed
smoothly, the pathogenesis of this common childhood vasculitis will be much clear,
and that are very important in the prevention and the treatment of this disease.
Subjects
過敏性紫斑症
內皮細胞
自體抗體
自體抗原
SDGs
Publisher
臺北市：國立臺灣大學醫學院小兒科
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
922314B002223.pdf
Size
129.27 KB
Format
Adobe PDF
Checksum
(MD5):14c6f10395151b422afc49d63398f3ab