利用B型肝炎-昆蟲桿狀重組病毒以偵測急性感染時肝細胞的基因表現

Abstract

Background/Aim: The hepatocyte gene expression profile at the time they respond to hepatitis B virus (HBV) acute infection was rarely studied. We aimed to describe this profile, which may help to delineate signal transduction pathway when virus enters inside the hepatocytes. Methods:We adapted baculo-HBV recombinant virus system to transfect HBV into non-primate, mammalian hepatocytes. This system was used to infect rat hepatocytes with HBV through portal vein injection of baculo-HBV recombinant virus. The control was the rat undergoing the same procedure but with the baculovirus that did not contain HBV. The hepatocyte RNAs of both rats were extracted for microarray and made for comparison. An in vitro study was done simultaneously. We also adapted the baculo-HBV recombinant virus and the baculovirus without HBV insert as the control to infected HepG2 cell cultures. The hepatoma cell RNAs of both cultures were also extracted for microarray and compared. Results: There were three differently expressed genes commonly found in both in vivo and in vitro studies: Stat1, Zinc finger protein 83 (ZNF83), and RNA binding motif protein 3 (RBM3). Conclusion: In the future, these genes of interest will become our foci to study their roles in the acute phase of HBV-hepatocyte interaction. Obviously, they are key cellular factors to allow the replication of HBV inside the hepatocytes. Based on these findings, we may develop antiviral therapies by targeting these factors.