利用B型肝炎-昆蟲桿狀重組病毒以偵測急性感染時肝細胞的基因表現
Date Issued
2005
Date
2005
Author(s)
倪衍玄
DOI
932314B002128
Abstract
Background/Aim: The hepatocyte gene expression profile at the time they respond to
hepatitis B virus (HBV) acute infection was rarely studied. We aimed to describe
this profile, which may help to delineate signal transduction pathway when virus
enters inside the hepatocytes. Methods:We adapted baculo-HBV recombinant virus
system to transfect HBV into non-primate, mammalian hepatocytes. This system
was used to infect rat hepatocytes with HBV through portal vein injection of
baculo-HBV recombinant virus. The control was the rat undergoing the same
procedure but with the baculovirus that did not contain HBV. The hepatocyte RNAs
of both rats were extracted for microarray and made for comparison. An in vitro
study was done simultaneously. We also adapted the baculo-HBV recombinant virus
and the baculovirus without HBV insert as the control to infected HepG2 cell cultures.
The hepatoma cell RNAs of both cultures were also extracted for microarray and
compared. Results: There were three differently expressed genes commonly found
in both in vivo and in vitro studies: Stat1, Zinc finger protein 83 (ZNF83), and RNA
binding motif protein 3 (RBM3). Conclusion: In the future, these genes of interest
will become our foci to study their roles in the acute phase of HBV-hepatocyte
interaction. Obviously, they are key cellular factors to allow the replication of HBV
inside the hepatocytes. Based on these findings, we may develop antiviral therapies
by targeting these factors.
Subjects
hepatitis B virus
baculo-HBV recombinant virus
microarray
Stat1
Zinc
finger protein 83
finger protein 83
RNA binding motif protein 3
SDGs
Publisher
臺北市：國立臺灣大學醫學院小兒科
Type
report
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