https://scholars.lib.ntu.edu.tw/handle/123456789/194708
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 小兒科 | en |
dc.contributor.author | CHIANG, BOR-LUEN | en |
dc.creator | 江伯倫 | zh-tw |
dc.creator | CHIANG, BOR-LUEN | en |
dc.date | 1998 | en |
dc.date.accessioned | 2008-12-10T08:37:27Z | - |
dc.date.accessioned | 2018-07-11T17:51:59Z | - |
dc.date.available | 2008-12-10T08:37:27Z | - |
dc.date.available | 2018-07-11T17:51:59Z | - |
dc.date.issued | 1998 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/90381 | - |
dc.description.abstract | It has been well demonstrated that interleukin-12 (IL-12) could be useful to defend against a variety of pathogens, to suppress tumor growth and metastasis, and even to be employed as an adjuvant of vaccines to enhance beneficial type 1 T helper (Th1) cell response over detrimental type 2 T helper (Th2) cell responses. To apply IL-12 genes in gene therapy such as a DNA vaccine, a pIL-12 vector was constructed that contained two cytomegalovirus (CMV) promoters to drive the expression of p35 and p40 subunits, respectively. In addition, a pscIL-12 vector was designed with a linker to fuse p35 cDNA with p40 cDNA to produce a single-chain IL-12 protein, ensuring not only that the expression of p35 and p40 subunits was equally expressed, but also that no free p40 subunits interfered with IL- 12 activity. The data suggested pIL-12 could produce a rather high level of biologically active IL-12 after transfection of COS cell lines as well as C2C12 muscle cell lines, as measured by both concanavalin A blast proliferation assay and enzyme-linked immunosorbent assay. Interestingly, the pscIL-12 vector could also express a bioactive murine IL-12 fusion protein in vitro. Furthermore, in vivo functional studies also demonstrated that mice co-immunized with a pS vector expressing the major envelope protein of hepatitis B virus (HBV) and IL-12 vectors encoding native IL-12 or single-chain IL-12 fusion protein elicited higher levels of IgG(2a) anti-HBs antibody and of Th1-related cytokine. Because p35 and p40 genes can be expressed in a vector by using a single promoter, pscIL- 12 should be useful in future applications for nucleic acid vaccination or for gene therapy against diseases. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | HUMAN GENE THERAPY v.9 n.4 pp.457-465 | en |
dc.relation.ispartof | HUMAN GENE THERAPY | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Construction of Vectors Expressing Bioactive Heterodimeric and Single- Chain Murine Interleukin-12 for Gene Therapy | en |
dc.type | journal article | en |
dc.relation.pages | 457-465 | - |
dc.relation.journalvolume | v.9 | - |
dc.relation.journalissue | n.4 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
顯示於: | 醫學系 |
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