Differential Integration Rates of Hepatitis B Virus DNA in the Liver of Children with Chronic Hepatitis B Virus Infection and Hepatocellular Carcinoma
Resource
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY v.20 n.8 pp.1206-1214
Journal
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
Journal Volume
v.20
Journal Issue
n.8
Pages
1206-1214
Date Issued
2005
Date
2005
Author(s)
CHANG, MEI-HWEI
HSU, HONG-YUAN
CHEN, HUEY-LING
JENG, YUNG-MING
NI, YEN-HSUAN
TSUEI, DAW-JEN
HUANG, HSIANG-PO
Abstract
Background and Aim: Integration of hepatitis B virus-DNA ( HBV-DNA) into the host genome, a phenomenon found frequently in hepatocellular carcinomas (HCC) and causally linked to oncogenesis, has not been well characterized in children. The aim of the present study was to determine the prevalence of HBV integration more accurately and to decide whether the integration rate varies at different stages of chronic HBV infection in children. Methods: Of 13 children with chronic hepatitis, 14 liver biopsy tissues were analyzed. One liver tissue with pure liver cirrhosis, nine non-tumor, and nine tumor liver tissues from children with HCC were analyzed by a very sensitive method, inverse polymerase chain reaction ( IPCR). Results: Thirteen genuine viral-host junctional sequences from 23 patients were successfully isolated and proved that IPCR is a useful method in this context. The results also indicated that the detection rate of HBV-DNA integration increased in parallel with the progress of liver histology towards the neoplastic transformation, with 0% in the liver of chronic hepatitis, 22.2% in non-tumor livers of HCC patients, and 66.7% in tumor liver tissues of HCC patients. Conclusion: The present results indicate that integration of HBV-DNA into the host genome was rarely confirmed at the early stage of chronic hepatitis in children until the stage of HCC formation.
Subjects
chronic hepatitis
hepatitis B virus
hepatocellular carcinoma
integration
inverse polymerase chain reaction
SDGs
Type
journal article