https://scholars.lib.ntu.edu.tw/handle/123456789/194863
標題: | Dendritic Cells Pulsed with Apoptotic Cells Activate Self-Reactive T- Cells of Lupus Mice Both in Vitro and in Vivo | 作者: | SUEN, JAU-LING CHIANG, BOR-LUEN |
關鍵字: | Apoptosis;Autoantigens;CD4þ T-cell;Dendritic cell;SL | 公開日期: | 2006 | 卷: | v.45 | 期: | n.10 | 起(迄)頁: | 1230-1237 | 來源出版物: | RHEUMATOLOGY | 摘要: | Objectives. Systemic lupus erythematosus (SLE) is characterized by the presence of autoantibodies (autoAbs) directed against the nuclear structure. Previous studies have demonstrated that dendritic cells (DCs) can process and present self-antigens (Ags) from apoptotic cells (ACs) in lupus. However, there is no direct evidence demonstrating that ACs provide self-Ags, such as histones, to stimulate autoreactive T-cells in lupus. Methods. AC-pulsed bone marrow-derived DCs (AC-BMDCs) were used to stimulate autoreactive T-cells in vitro and in vivo. Results. In our study , we found that AC-BMDCs could induce the proliferation of CD4(+) T-cells from unprimed NZB x NZW F1 (BWF1) mice, which spontaneously develop SLE, but not CD4(+) T-cells, from non-autoimmune DBA-2 x NZW F1 (DWF1) mice. In addition, AC-BMDCs could induce significant proliferative responses to certain histone peptide-specific T-cells. Furthermore, these AC-BMDCs could induce a considerable anti-DNA Ab response in vivo after adoptive transfer into DWF1 mice, suggesting that AC-BMDCs can break tolerance in normal mice and initiate an autoimmune response. Conclusion. Our study provides a direct link between self-epitopes from ACs presented by DCs and autoreactive T-cell activation, and demonstrates that ACs are critical for the induction of autoimmunity in vivo. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/92619 |
顯示於: | 醫學系 |
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