|Title:||Decreased Diversity of Hepatitis C Virus Quasispecies during Bone Marrow Transplantation||Authors:||NI, YEN-HSUAN
|Keywords:||hepatitis C virus;quasispecies;bone marrow transplantation||Issue Date:||1999||Journal Volume:||VIROLOGY||Journal Issue:||n.2||Start page/Pages:||132-138||Source:||JOURNAL OF MEDICAL||Abstract:||
To elucidate the role of host immune status in the evolution and complexity of hepatitis C virus (HCV) quasispecies, three chronic HCV- infected patients who underwent bone marrow transplantation (BMT) were studied. The three transplanted patients' sera were sampled at pre-BMT, 3 months after BMT, and 12 months after BMT and the nucleotide diversity and substitution of the hypervariable region (HVR ) of HCV quasispecies were analyzed. The nucleotide diversity was high at the pre-BMT period (28.2-43 .4 x 10-2 nucleotide difference/site). HVR of HCV quasispecies then became homogeneous in the first 3 months after BMT (0.116.40 x 10-2 nucleotide difference/site). The nucleotide diversity of HVR at 12 months after BMT of all three patients was higher than that of 3 months after BMT but still lower than that of pre-BMT of all three patients was higher than that 3 months after BMT but still lower than that of pre-BMT (2.09-6.40x10-2 nucleotide difference/site). The analysis on nucleotide substitution rate showed a hisher value between pre-BMT and 3 months after BMT (0.624-0 .708 nucleotide difference/site per year)than that between 3 months and 12 months after BMT (0.0720.127 nucleotide difference /site per year). HCV RNA titer decreased when the host had a low white cell count and increased accordingly. It was concluded that the evolution of HVR of HCV quasispecies related to the immune status of the host during BMT : after immunosuppression, an initial increase of viral populations was followed by the emergence of a dominant strain while the quasispecies qradually recovered as the immunity of the host gained its competence.
|Appears in Collections:||醫學系|
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