Expression of Caspid Protein Vp1 for Use as Antigen for the Diagnosis of Enterovirus 71 Infection
Resource
JOURNAL OF MEDICAL VIROLOGY v.61 n.2 pp.228-234
Journal
JOURNAL OF MEDICAL
Journal Volume
VIROLOGY
Journal Issue
n.2
Pages
228-234
Date Issued
2000
Date
2000
Author(s)
SHIH, SHIN-RU
LIN, TZOU-YIEN
CHANG, LUAN-YIN
HUANG, YHU-CHERING
TSAO, KUO-CHIEN
NING, HSIAO-CHEN
Abstract
To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a poly -histidine fusion protein. Western blotting experiments with sera from patients with enterovirus 71 infection indicated that immunoglobulin G (IgG) and IgM antibodies bound to a single polypeptide VP1. According to these results, IgM anti -VP1 appeared in sera of patients with a symptomatic enterovirus 71 acute infection, whereas IgG anti-VP1 was present in sera of past infection. This finding suggests that detecting IgG and IgM immune responses against linear epitopes of recombinant VP1 is an effective means of determining the different phases of enterovirus 71 infection . In addition, sera containing coxsackie virus 16 ( CA16) antibodies did not cross-react with the recombinant VP 1 of enterovirus 71, despite the homology between VP1 proteins of both viruses. Comparison with reference PCR and neutralization assays showed these antibody tests to be appropriate for the serodiagnosis of enterovirus 71 infection.
Subjects
enterovirus 71
recombinant capsid protein VP1
serodiagnosis
acute infections
past infections
enterovirus 71 antibody
Type
journal article