https://scholars.lib.ntu.edu.tw/handle/123456789/194923
標題: | Functional Measurement of Hepatitis C Virus Core-Specific Cd8+T-Cell Responses in the Livers or Peripheral Blood of Patients by Using Autologous Peripheral Blood Mononuclear Cells as Targets or Stimulators | 作者: | FANG, SHIH-HUA CHIANG, BOR-LUEN WU, MEI-HUA LAI, MING-YANG YANG, PEI-MING CHEN, DING-SHINN HWANG, LIH-HWA |
關鍵字: | VESICULAR STOMATITIS-VIRUS;CYTOTOXIC T-LYMPHOCYTES;MEDIATED GENE-TRANSFER;PSEUDOTYPED RETROVIRAL VECTORS;CRE RECOMBINASE;HIGH-TITER | 公開日期: | 2001 | 卷: | v.39 | 期: | n.11 | 起(迄)頁: | 3895-3901 | 來源出版物: | JOURNAL OF CLINICAL MICROBIOLOGY | 摘要: | As is widely recognized, CD8(+) cytotoxic T lymphocytes ( CTLs) play a crucial role in hepatitis C virus (HCV) infection, both in pathogenesis of liver injury and in clearing the virus. CTL studies with HCV-infected patients have been difficult because of the relatively low frequency of CTL precursors in the peripheral blood and because the targeted epitopes vary depending on the human leukocyte antigen (HLA) types of the individuals. This study attempts to overcome these problems by assessing the feasibility of using autologous peripheral blood mononuclear cells ( PBMCs) expressing viral antigens as stimulators or targets in order to monitor the CTL responses. Primary PBMCs were transduced using a retroviral vector pseudotyped with a vesicular stomatitis virus G glycoprotein expressing the HCV core gene . Additionally, the vector- transduced PBMCs were used as targets of CTL assays to measure the HCV core-specific CTL activities from the liver-infiltrating lymphocytes of six different HLA-type patients with chronic HCV infection. The core- expressing PBMCs also served as stimulators, allowing us to measure core- specific CD8+ T-cell responses by intracellular gamma interferon staining of the peripheral blood of hepatitis C patients who had received treatment with alpha interferon plus ribavirin. This approach provides an efficient means of measuring antigen-specific CTL responses without HLA constraints . |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94815 |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。