https://scholars.lib.ntu.edu.tw/handle/123456789/194947
Title: | Interleukin-12 Inhibits Eotaxin Secretion of Cultured Primary Lung Cells and Alleviates Airway Inflammation in Vivo | Authors: | CHIANG, BOR-LUEN | Keywords: | eotaxin;interferon-gamma;interleukin-12;lung cell culture | Issue Date: | 2002 | Journal Volume: | v.19 | Journal Issue: | n.2 | Start page/Pages: | 76-84 | Source: | CYTOKINE | Abstract: | The mechanisms that cause the inflammation of airway and lung tissue in asthma have been studied extensively. It is noted that type 1 T helper cell (Th1)-related cytokines could decrease the accumulation of eosinophils in lung tissue and relieve airway constriction. But the therapeutic mechanisms of Th1 cytokines remain unclear. In this study, interleukin-12 (IL-12) DNA plasmid as a therapeutic reagent was delivered intravenously. Bronchoalveolar lavage (BAL) fluids were collected from IL- 12 treated and control mice, and analyzed for cell composition and eotaxin level. The results showed that IL-12 DNA plasmid could effectively inhibit eosinophilia and airway inflammation in vivo. The level of eotaxin in BAL fluid also decreased. To further investigate the effect of Th1- related cytokines, such as IL- 12 or interferon-gamma (IFN-gamma) on the eotaxin level produced by lung cells, primary lung cell culture was established. The results demonstrated that both IL-12 and IFN-gamma could suppress eotaxin secretion from IL-13 or IL- 4 stimulated primary lung cell culture. Moreover, the inhibitory effect of IL-12 could not be reversed by the administration of anti-IFN-gamma antibody. All the evidences suggested that IL-12 could regulate airway inflammation by suppressing the eotaxin secretion of lung tissue through an IFN-gamma independent mechanism. (C) 2002 Elsevier Science Ltd. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94839 |
Appears in Collections: | 醫學系 |
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