https://scholars.lib.ntu.edu.tw/handle/123456789/194950
標題: | Study of an Outbreak of Enterobacter Cloacae Sepsis in a Neonatal Intensive Care Unit: The Application of Epidemiologic Chromosome Profiling by Pulsed-Field Gel Electrophoresis | 作者: | LEE, PING-ING | 關鍵字: | GENOMIC RESTRICTION FRAGMENTS;NOSOCOMIAL ENTEROBACTER;DNA;INFECTION | 公開日期: | 2002 | 卷: | v.30 | 期: | n.7 | 起(迄)頁: | 381-385 | 來源出版物: | AMERICAN JOURNAL OF INFECTION CONTROL | 摘要: | Objective: From October 1996 to March 1997, a cluster of II cases of neonatal sepsis caused by Enterobacter cloacae with similar antimicrobial susceptibility patterns occurred in a neonatal intensive care unit. This outbreak prompted an investigation. Method: Twelve isolates obtained from 6 neonatal patients who developed E cloacae sepsis during the outbreak were analyzed. Four E cloacae isolates from 2 preterm neonates without E cloacae infection on the same ward, and I isolate from the hands of a nurse, were also examined. No E cloacae were isolated from the environment . Bacterial DNA digested with XbaI or NotI was analyzed with pulsed-field gel electrophoresis. Result: Three distinct banding patterns were identified by pulsed-field gel electrophoresis. Of the 6 preterm infants with sepsis, strain I was identified in I, strain II in 2, a mixed infection of strains I and II in 2, and strain III was found in only I infant. An isolate from the hands of a nurse was identified as strain II, as were the 4 isolates from the 2 preterm neonates without E cloacae infection. Thus, this outbreak of sepsis was caused by 2 genotypes of E cloacae. Conclusion: This study demonstrates that pulsed-field gel electrophoresis with restriction enzyme digestion is a valuable tool for genetic characterization of multidrug- resistant E cloacae strains during outbreaks. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94842 |
顯示於: | 醫學系 |
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