|Title:||Identification of T-Cell Epitopes on U1a Protein in Mrl/Lpr Mice: Double- Negative T Cells Are the Major Responsive Cells||Authors:||Yang, Mei-Hui
|Keywords:||SYSTEMIC-LUPUS-ERYTHEMATOSUS;DENDRITIC CELLS;LPR MICE;IN- VIVO;AUTOANTIBODIES;AUTOIMMUNITY||Issue Date:||2005||Journal Volume:||v.115||Journal Issue:||n.2||Start page/Pages:||279-286||Source:||IMMUNOLOGY||Abstract:||
Systemic lupus erythematosus (SLE) is characterized by the existence of a heterogeneous group of autoantibodies such as anti-DNA, chromatin, histone, and ribonucleoprotein antibodies (Abs). Although the B-cell antigenic determinants have been well characterized, very limited data about the T -cell epitopes of self-antigen (Ag) have been reported. In the present study, we analysed auto-T-cell epitopes using bone marrow-derived dendritic cells (BM-DCs) pulsed with murine U1A (mU1A) protein capable of activating autoreactive T cells from unprimed MRL/lpr mice in vitro. The data suggested that there are at least four T-cell epitopes on the U1A protein, U1A3150, U1A6180, U1A201220 and U1A271287, and U1A3150 had the most significant T-cell proliferative response. In addition, the main responsive T cells are the CD4 CD8 double-negative subgroup of T cells. Furthermore, we also demonstrated that the activation of double-negative T cells is major histocompatibility complex class II restricted. The study here provides information on T-cell epitope analysis of the U1A antigen using BM-DCs as the effective antigen-presenting cells.
|Appears in Collections:||醫學系|
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