|Title:||Identification and Expression of Pen C 2, a Novel Allergen from Penicillium Citrinum||Authors:||CHOW, Lu-Ping
|Keywords:||allergy;cDNA cloning;IgE-binding activity;mould allergens;vacuolar serine protease;GROUP-III ALLERGEN||Issue Date:||1999||Journal Volume:||v.341||Journal Issue:||n.PART1||Start page/Pages:||51-59||Source:||BIOCHEMICAL JOURNAL||Abstract:||
The mould genus, Penicillium, is known to be a significant source of environmental aero-allergens. One important allergen from Penicillium citrinum, Pen c 2, has been identified by means of two-dimensional immunoblotting using IgE-containing patients' sera. This novel allergen was cloned, sequenced and expressed in Escherichia toll. The cloned cDNA encodes a large 457-amino acid protein precursor containing a 16-amino acid signal peptide, a 120-amino acid propeptide and the 321-amino acid mature protein. Comparison of the Pen c 2 sequence with known protein sequences revealed shared high sequence similarities with two vacuolar serine proteases from Aspergillus niger and Saccharomyces cerevisiae. Asp- 36, His-78 and Ser-244 were found to constitute the catalytic triad of the 39-kDa Pen c 2, The DNA coding for Pen c 2 was cloned into vector PQE-30 and expressed in E. coli as a His-tag fusion protein that bound serum IgE from Penicillium-allergic patients on immunoblots, Recombinant Pen c 2 could therefore be used effectively for diagnosis and also potentially for the treatment of mould-derived allergic disorders.
|Appears in Collections:||醫學系|
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