https://scholars.lib.ntu.edu.tw/handle/123456789/195583
Title: | Defective Functions of Circulating Cd4+Cd25+ and Cd4+Cd25- T Cells in Patients with Chronic Ordinary Urticaria | Authors: | CHEN, WU-CHARNG CHIANG, BOR-LUEN LIU, HSING-JIN EUGENE LEU, SY-JYE LEE, YUEH-LUN |
Keywords: | chronic ordinary urticaria;CD4+CD25+ regulatory T cells;FOXP3;suppressive function;CD4+CD25- T cells;cytokines | Issue Date: | 2008 | Journal Volume: | v.51 | Journal Issue: | n.2 | Start page/Pages: | 121-130 | Source: | JOURNAL OF DERMATOLOGICAL SCIENCE | Abstract: | BACKGROUND: Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50% have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4(+)CD25(+) regulatory T ( Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU. OBJECTIVE: To identify whether CD4(+) T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4(+)CD25(+) and CD4(+)CD25( -) T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4( +)CD25(+) T cells. METHODS: Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4(+)CD25(+) T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP 3) and transforming growth factor-beta( TGF-beta) in CD4(+)CD25(+) T cells were detected by real- time PCR. Furthermore, the suppressive function of CD4(+)CD 25(+) T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4(+)CD25( -) T cells was measured by ELISA. RESULTS: An increased frequency of CD4(+)CD25(+) T cells was observed in CU patients (n=19) compared to control subjects (n=7 ). No significant difference was detected in the expression levels of FOXP 3 or TGF-beta between CU patients (n=14) and control subjects (n=7). Strikingly, the suppressive capacity of CD4 (+)CD25(+) Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4(+ )CD25(-) T cells was significantly reduced in CU patients (n =9) compared to healthy donors (n=11). CONCLUSIONS: Our data demonstrate that CD4(+)CD25(+) and CD4(+)CD25(-) T cells in PBMCs exhibit defective functions in CU patients. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/186763 |
Appears in Collections: | 醫學系 |
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