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  4. Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional in Vitro Fertilization
 
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Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional in Vitro Fertilization

Resource
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS v.21 n.10 pp.367-369
Journal
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
Journal Volume
v.21
Journal Issue
n.10
Pages
367-369
Date Issued
2004
Date
2004
Author(s)
CHEN, SHEE-UAN
LIEN, YIH-RON
CHANG, LI- JUNG
TSAI, YI-YI
HO, HONG-NERNG
YANG, YU-SHIH
URI
http://ntur.lib.ntu.edu.tw//handle/246246/99411
Abstract
Purpose: To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection ( ICSI ) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle. Methods: The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed. Results: After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX. Conclusions: In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.
Subjects
cryopreserved oocytes
ntracytoplasmic sperm injection
unexpected fertilization failures

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