https://scholars.lib.ntu.edu.tw/handle/123456789/197914
Title: | 行政院國家科學委員會專題研究計畫成果報告:轉綠因子NF-KB與AP-I於神經肌肉疾病中所扮演的角色 | Authors: | 楊智超 | Keywords: | transcription factor;NF-κB;AP- 1;immunocytochemistry;neuromuscular disease;轉錄因子;AP-l;免疫組織化學;神經肌肉疾病 | Issue Date: | 1999 | Publisher: | 臺北市:國立臺灣大學醫學院神經科 | Abstract: | Immunohistochemical study of subunits of transcription factor NP- i B and AP-l in various neuromuscular disease showed: (1) A majority of necrotic fibers had diffuse immunoactivities(IR) of p50; ER of p65 was only present in a subset. Most of the p50 JR did not co-localize with macrophages; coexistence of p65 and macrophage JR was seen occasionally.(2)In 20-30% of regenerating fibers, there were diffuse p50 and p65 JR. (3) No increase of NP- K B or AP-l JR was observed in neurogenic disorders. (4) For AP-1 IR, c-jun was increasd in a subset of regenerating fibers but not in necrotic fibers. JR of c-fos was not detected in all biopsies. Our results demonstrate that NF- K B and c-jun can be increascd in human muscle disease. Different distribu ion of NF- K B and AP-1 subunits points to their selective role in muscle patholoy. 我們使用光學顯微鏡免疫組織化學的方法來觀察轉錄因子NF-κB及AP-1在正常肌肉及各種神經肌肉疾病的分佈情形。所使用的抗體包括NF-κB次單位PSO 、P65 的抗體與AP-l 次單位c-Jun 及c-Fos 的抗體。大部分的壞死細胞具有廣泛且強烈的p50 免疫活性。只有一部分的壞死細胞具有p65 的免疫活性。這些免疫活性並非存在於巨噬細胞內。在20 -30 %的再生細胞中可觀察到P65 及p50 的免疫活性。AP-1中的c-Jun 免疫活性在一部分的再生細胞中有增加的現象,不過在壞死細胞中並不增加。C-fos 的免疫活性並沒有觀察到有明顯的變化。在神經源性疾病不管AP-l 或NF-κB均無明顯變化。AP-l 或NF-κB在肌肉的壞死及再生可能扮演著一重要的角色。不同的次單元也具有不同的地位。 |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/26075 | Other Identifiers: | 882314B002292 | Rights: | 國立臺灣大學醫學院神經科 |
Appears in Collections: | 醫學系 |
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882314B002292.pdf | 270.16 kB | Adobe PDF | View/Open |
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