行政院國家科學委員會專題研究計畫成果報告:實驗性自體免疫前葡萄膜炎之基因治療
Date Issued
1999
Date
1999
Author(s)
林昌平
DOI
882314B002376M47
Abstract
Experimental autoimmune anterior uveitis (EAAU) is an inflammatory autoimmune disease of anterior uveal tract, which serves as a iodel for human acute anterior uveitis. It has been shown that EAAU is mediated by CD4+ T cells and delayed type hypersensitivity, We have shown that intraocular injection of soluble melanin associated antigen can prevent the development cf primary onset and recurrence of
EAAU.
To test the possibility of gene therapy for EAAU, we will inject IL-4 plasmid entrapping liposome and bovine MAA (uveitoger ic antigen in the model of EAAU) into the hind footpad of Lewis rat. Our rational is the injected liposome-entrapped IL- 4 plasmid will be taken to regional lymph node ar d transfect the lymphoid cells and antigen presenting cells over there. This will develcip an IL-4 rich microenvironment that favors T helper 2 (Th2) response when antigen is presented to and activate specific T cell clones. T helper 2 pattern of cytoki e secretion will downregulate T helper 1 (Thi) response and delayed type hypersensitivity and therefore prevent and/or attenuate the severity of the autoimmune inflammatory disease. The liposome entrapped plasmid will stay longer in tissue and al c easier to be taken along with lymph to regional lymph node, and will make this gene therapy last longer and more effective.
This gene therapy will only involve the use of tiny amount of nucleic acid, and will avoid large amount of systemic administration of cytokines to modulate immune response. The injection of the mixture of antigen and nucleic acid at the same site can make this immunomodulation more antigen specific. This approach could be called ¡¥therapeutic vaccine¡¦, If it works and can be applied to uveitis patients, it will be of great benefit to them, who usually suffer from repe ited recurrences and various complications that will lead to blindness.
Subjects
Uveitis
autoimmune disease
liposOme
interleukin
vaccine
SDGs
Publisher
臺北市：國立臺灣大學醫學院眼科
Type
journal article
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