Application of irradiation in limb allo-transplantation , a rat model
Date Issued
1998-07-31
Date
1998-07-31
Author(s)
雷德
DOI
872314B002002
Abstract
Following whole limb transplantation from
Brown-Norway (BN) to Lewis (LEW) rats,
Graft-vs-Host Disease (GVHD) is observed
after the administration of
immunosuppressive agents is discontinued.
In this study, lethal donor irradiation and
variation in immunosuppressive schedules
were studied in an attempt to prevent GVHD.
In group one (n=4, control), all BN limbs
were rejected by untreated LEW recipients in
7.5±0.3 days after transplantation.
Combination cyclosporine (5 mg/kg/day) and
leflunomide (10 mg/kg/day) were given
orally to 3 additional groups of LEW
recipients until 60 days following
transplantation. In group 2 (n=6), drug
treatment began 2 days prior to surgery. Over
days 63 to 65, all recipients developed signs
of GVHD. The limbs survived for 75.6±3.3
days. In group 3 (n=11), drug treatment
began on the day of surgery. All but one rat
developed signs of GVHD , comparable to
that seen in group 2 , over days 63 to 65.
The mean limb survival time was 83.6±5.7
days. In group 4 (n=6) donor rats were
treated with total body irradiation (15 Gy) 2
days before surgery. Drug treatment of the
LEW recipients began 2 days prior to surgery.
Three of the 6 rats developed GVHD
following discontinuation of drug treatment,
and the mean limb survival time was
73.2±0.9 days. The incidence of GVHD
was not significantly different (p=0.09,
Fisher’s exact test) among the 3 treated
groups. These results show that neither
lethal donor total body irradiation or
temporal variation in the administration of
immunosuppressive agents can abolish
GVHD in this model of whole limb
allotransplantation.
Subjects
limb transplantation
GVHD
total body irradiation
Publisher
臺北市：國立臺灣大學醫學院放射線科
Type
journal article
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