不同薄膜之表面特性與生物適應性之影響

Abstract

Monocyte adhesion and subsequent activation are major events that facilitate the foreign-body reaction. These studies evaluate the effect of semicrystalline polyamide (Nylon-66), poly (ethylene-co-vinyl alcohol) (EVAL), and poly (vinylidene fluoride) (PVDF) with nonporous and porous morphologies on the ability of monocyte adhesion and activation to produce variable levels of IL-1b, IL-6, and TNF-a. Results indicated IL-1b was produced in the greatest quantity by these polymers. In addition, monocyte adhesion and activation on a material may alter to a great extent dependent on the surface morphology and wettability. As the membrane wettability increases, the ability of the membrane to adhere monocytes increases but to stimulate monocyte production of cytokines decreases. Similarly, these membranes when prepared with porous surfaces can enhance monocyte adhesion and suppress monocyte activity. Therefore, the nonporous PVDF membrane is the least biocompatible in this work. In contrast, the hydrophilic membrane Nylon-66 with porous surface is the least stimulating of monocyte cytokine production when compared to all of the other membranes evaluated with nonporous or porous surface. These studies provide important insight into conditions that modulate monocyte activity in response to the substratum morphology and wettability.