惡性肋膜積液中各種細胞之結合因子表現

Abstract

背景：癌細胞表面之各種結合因子與腫瘤 細胞之侵襲性和轉移能力有密切之關係， 轉移至肋膜積液中各種細胞上的結合因子 表現未曾見諸文獻。 目的︰為觀察惡性肋膜積液中各種良性細 胞與惡性細胞表面之結合因子表現。 實驗設計︰收集15 例經病理學和細胞學證 實且有癌細胞存在的惡性肋膜積液，以細 胞離心法製作抹片，然後依ABC peroxidase 法進行免疫染色，以顯微鏡計數各類細胞 具結合分子表現之百分率。 結果︰癌細胞表面之結合因子以CD44 (64%)，E-cadherin (60%)為最常見；而中皮 細胞則以CD44 (72%)和VLA-4 (57%)表現 為最多，其餘其中白血球則以CD44 (72%) 和LFA-1 (71%)為最多。 討論︰以往離體試驗發現具有CD44 和Ecadherin 之癌細胞較具侵襲性和轉移能 力，肋膜積液中癌細胞是否是原發腫瘤中 較具侵襲性之細胞群？本研究發現人體內 侵入肋膜腔內之癌細胞也以具有CD44 和 E-cadherin 表現之細胞為多數，似乎印證以 往之離體觀察。 評論︰本研究首次證明肋膜積液中各種癌 細胞具有較高之CD44和E-cadherin二種結 合因子。各種細胞間之互動是否與結合因 子表現有關？由於技術上之困難而無法充 分清楚顯現而難以判斷。

Background: The expression of adhesion molecules on the surface of tumor cells has been found to be related to the invasiveness and metastasis of the neoplasm. The role of adhesion molecules on the cells of malignant pleural effusion has not been reported in the literature. Purposes: To observe the expression of adhesion molecules on the cells in malignant pleural effusion. Design: Fifteen histologically/cytologically verified malignant pleural effusions were enrolled in this study. The cells prepared with cytospin were stained with a pannel of adhesion molecules by ABC peroxidase method. The percentage of cells with expression of adhesion molecules were counted with a microscope. Results: The most common adhesion molecules on the cancer cells are CD44 (64%) and E-cadherin (60%); on mesothelial cells are CD44 (72%) and VLA-4 (57%). Those on leukocytes are CD44 (72%) and LFA-1 (71%). Discussion: Previous in vitro study revealed the cancer cells with expression of CD44 and E-cadherin were more invasive & metastatic. This study showed that the cancer cells in pleural effusion had high percentage of cells with CD44 and Ecadherin. This study confirmed the previous observation in different malignant cells in vivo. Comment: This is the first in vivo study that confirmed the malignant cells in pleural effusions expressied adhesion molecules of CD44 and E-cadherin. Due to technical difficulty, the role of adhesion molecule in the cell-to-cell interaction could not be clearly demonstrated.