Synergistic Interaction of Angiotensinogen and Angiotensin-conver ting Enzyme Genes in Predicting Coronary Ar ter iosclerosis
Date Issued
1999-07-31
Date
1999-07-31
Author(s)
Chiang, Fu-Tien
DOI
882314B002319
Abstract
Angiotensinogen (AGT) and angiotensin-converting enzyme (ACE) gene
polymorphisms have been implicated in the pathogenesis of coronary artery disease
(CAD) and myocardial infarction (MI). However, conclusions are inconsistent and
there are few combination analysis of the AGT and ACE genes in relation to CAD
and MI. We therefore study 255 Taiwanese people, including 171 CAD patients and
84 non-CAD controls. A novel stepdown polymerase chain reaction (PCR) was used
to genotype the ACE insertion/deletion(I/D) polymorphism and a stepdown PCRdirect
sequencing to genotype the M235T and T174M variants of the AGT gene.
The results showed that the 235T allele and TT genotype frequencies of the AGT gene
were 82% and 65% respectively in the non-CAD group vs. 83% and 68% in the CAD
group (p>0.05). The 174T allele and TT genotype frequencies were 92% and 86% in
the non-CAD group vs. 89% and 80% in the CAD group (p>0.05). The D allele and
DD genotype frequencies of the ACE gene were 64% and 40% respectively in the
non-CAD group vs. 69% and 46% in the CAD group (p>0.05). There was no
difference of AGT or ACE gene polymorphism in subjects with MI, low risk, or
different severities of CAD. However, subjects with double homozygosity of
235TT/DD genotype had a higher prevalence (75%) of CAD than those with
235TT/(ID+II) (52%) or (235MT+MM)/DD (56%). The odds ratio was 2.61 (95%
CI 1.88 to 3.61). Subjects with double heterozygosity of (235MT+MM)/(ID+II) also
had a higher prevalence (71%) of CAD. The odds ratio was 2.14 (95% CI 1.48 to
3.09). Our data show that no association between each single gene polymorphism of
the AGT and ACE and coronary heart disease can be found in the Taiwanese
population. However, there is a synergistic gene effect that confers an increased risk
for CAD.
Subjects
Gene-gene interaction
Taiwanese
CAD
MI
Heterozygosity
Publisher
臺北市：國立臺灣大學醫學院檢驗醫學科
Type
journal article
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