Effect of Glutathione and DNA Methylation on Ar senic Resistance in Cancer Cells
Date Issued
2000-07-31
Date
2000-07-31
Author(s)
陳耀昌
DOI
892314B002113
Abstract
Arsenic is a ubiquitous element that
presents in environment. Chronic exposure
of arsenic compound is associated with
increased risk of skin cancer, lung cancer,
bladder cancer, etc. Arsenic is an ancient
medication and was used widely in both
western and Chinese medicine. It has been
used to treat acute promyelocytic leukemia
in the past few years. Arsenic has been used
in clinical trials involving other cancer
types.
Cellular glutathione system and DNA
hypomethylation has been linked to arsenic
toxicity to normal cells. Our preliminary
study on a panel of cancer cell lines
suggested that high cellular glutathione was
associated with arsenic resistance in cancer
cells. The objective of this project is to
correlate cellular glutathione level, DNA
methylation level with arsenic resistance in
cancer cells. In a panel of cell lines,
cytotoxicity to arsenic correlate well to
glutathione content in cancer cells. NTU-B1
cells that contain low level of glutathione
has lower level of global DNA methylation
than high glutathione containing arsenic
resistant NTU-B1/P14 cells. However, there
was no further DNA hypomethylation when
BSO were added to either NTU-B1 or NTU2
B1/P14 cells. There is no correlation
between arsenic sensitivity to global DNA
methylation. Arsenic sensitivity may be
related to only some specific gene
methylation. Depletion of glutathione by
BSO was not able to change global DNA
methylation in cancer cells.
Subjects
氧化砷
癌症治療
抗藥性
SDGs
Publisher
臺北市：國立臺灣大學醫學院檢驗醫學科
Type
journal article
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