DC 欄位 | 值 | 語言 |
dc.contributor.author | 陳耀昌 | zh_TW |
dc.creator | 陳耀昌 | zh_TW |
dc.date | 2000-07-31 | zh_TW |
dc.date.accessioned | 2006-07-26T04:53:34Z | en |
dc.date.accessioned | 2018-07-12T08:58:09Z | - |
dc.date.available | 2006-07-26T04:53:34Z | en |
dc.date.available | 2018-07-12T08:58:09Z | - |
dc.date.issued | 2000-07-31 | - |
dc.identifier | 892314B002113 | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/27327 | - |
dc.description.abstract | 砷元素廣泛的散佈在自然界中。長期的
暴露在過量的砷會增加得到皮膚癌、肺
癌、膀胱癌等癌症的機會。但自古以來,
砷就被西方醫學及傳統中醫用來治療多種
疾病,最近發現,氧化砷能治療對化學治
療、全反式維生素甲酸完全沒有效用的急
性前骨髓型白血病病人。砷也被應用在其
他癌症的臨床試驗中。
細胞排除砷的能力似乎和glutathione
系統有關,此外砷對細胞的毒性似乎和去
氧核糖核酸甲機化的程度有關。我們初期
研究顯示對氧化砷有抗藥性的癌細胞,
glutathione 的量較高,使用抗藥逆轉劑
buthionine sulfoximine (BSO) 降低
glutathione 可增加氧化砷的敏感度。
本計畫的目的在探討癌細胞抗氧化砷
和glutathione,及去氧核糖核酸甲機化的
關係。我們發現在一系列癌細胞中
glutathione 的量和氧化砷的毒性很有相
關。對氧化砷敏感的NTU-B1 細胞(低
glutathione)去氧核糖核酸甲機化的程度,
比對氧化砷有抗藥性的NTU-B1/P14(高
glutathione)為低。用BSO 處理NTUB1/
P14 細胞可增強氧化砷對癌細胞的毒
殺能力,但去氧核糖核酸甲機化的程度並
無改變。全細胞去氧核糖核酸甲機化的程
度應和氧化砷有抗藥性無關,也許只有和
特定基因的去氧核糖核酸甲機化有關。細
胞內glutathione 的改變也無法改變全細胞
去氧核糖核酸甲機化的程度。 | zh_TW |
dc.description.abstract | Arsenic is a ubiquitous element that
presents in environment. Chronic exposure
of arsenic compound is associated with
increased risk of skin cancer, lung cancer,
bladder cancer, etc. Arsenic is an ancient
medication and was used widely in both
western and Chinese medicine. It has been
used to treat acute promyelocytic leukemia
in the past few years. Arsenic has been used
in clinical trials involving other cancer
types.
Cellular glutathione system and DNA
hypomethylation has been linked to arsenic
toxicity to normal cells. Our preliminary
study on a panel of cancer cell lines
suggested that high cellular glutathione was
associated with arsenic resistance in cancer
cells. The objective of this project is to
correlate cellular glutathione level, DNA
methylation level with arsenic resistance in
cancer cells. In a panel of cell lines,
cytotoxicity to arsenic correlate well to
glutathione content in cancer cells. NTU-B1
cells that contain low level of glutathione
has lower level of global DNA methylation
than high glutathione containing arsenic
resistant NTU-B1/P14 cells. However, there
was no further DNA hypomethylation when
BSO were added to either NTU-B1 or NTU2
B1/P14 cells. There is no correlation
between arsenic sensitivity to global DNA
methylation. Arsenic sensitivity may be
related to only some specific gene
methylation. Depletion of glutathione by
BSO was not able to change global DNA
methylation in cancer cells. | en |
dc.format | application/pdf | en |
dc.format.extent | 58649 bytes | en |
dc.format.mimetype | application/pdf | en |
dc.language | zh_TW | zh_TW |
dc.language.iso | zh_TW | zh_TW |
dc.publisher | 臺北市:國立臺灣大學醫學院檢驗醫學科 | zh_TW |
dc.rights | 國立臺灣大學醫學院檢驗醫學科 | zh_TW |
dc.subject | 氧化砷 | zh_TW |
dc.subject | 癌症治療 | zh_TW |
dc.subject | 抗藥性 | zh_TW |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | GLUTATHIONE及去氧核糖核酸甲機化對癌細胞氧化砷抗藥性之影響 | zh_TW |
dc.title | Effect of Glutathione and DNA Methylation
on Ar senic Resistance in Cancer Cells | en |
dc.type | journal article | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/27327/1/892314B002113.pdf | - |
dc.coverage | 計畫年度:89
第一期;起迄日期:1999-08-01/2000-07-31 | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | zh_TW | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
顯示於: | 醫學系
|