高分子薄膜於外加電場下應用於血漿蛋白質分離的研究

Abstract

過去實驗已證實自製之高分子薄膜於 分離免疫球蛋白及白蛋白之可行性，惟其 間發現分離之效果未臻理想之最大原因為 蛋白質在薄膜表面形成一層濾餅（cake）， 為改進此缺失，本實驗乃以掃流過濾及外 加電場，以期藉流動性溶液及電場使免疫 球蛋白不致沉積在薄膜表面，阻塞過濾效 果，以便白蛋白通過薄膜，如此將可達到 預期的效果。故本實驗分為三部分：（1） 薄膜結構之鑑定包括薄膜之表面，截面， 孔隙度大小之測定（2）蛋白質溶液在外加 電場掃流之過濾實驗及（3）血液檢體於同 樣狀況下之測試。希望此外加電場掃流之 簡易裝置能徹底改善過去因阻塞造成阻力 增加，導致過濾效果降低之缺失，使此高 分子薄膜能作為臨床上治療性血漿分離時 二次過濾之用。

Previous experiments have proved that membranes have the possibility to separate of globulins and albumin. From these experiments, we found the pitfall causing the lower filtration efficiency is due to the “cake” formation on the membranes. In order to improve this pitfall, we try to add an electric field and cross-flow to the experiments. As we know, the anode will attract the negativechanged globulins with the cross-flow that will prevent the obstruction of the micropores. By the way, the cross-flow will simulate the condition of cascade filtration by its continuous flow. So, three parts will compose the studies: 1) Identification of membrane structures including surface and cross-section, measuring size of the micropores, contact angles and degree of crystallization. 2) Experiments of protein solutions filtered through membranes within a cross-flow device under electric field. 3) Experiments of plasma of different composition filtered through membranes under the same conditions of above. We hope this simple cross-flow and electric field design will improve the filtration efficacy and let the membrane can be used clinically in cascade or double filtration technique in therapeutic plasmapheresis.