Studies on the mechanism(s) of action of estrogen and environmental estrogens on the cell growth of osteoblast
Date Issued
2002-07-31
Date
2002-07-31
Author(s)
蔡克嵩
DOI
902314B002224
Abstract
It has been found that estrogen had
protective effects on bone, but the
mechanisms of action have not been
fully established. Environmental
estrogens include the detergents,
pesticide, chlorinated insecticides,
polycyclic aromatic hydrocarbons and
phytosterol esters, and some of these
compounds have recently been
reported to modulate sexual
differentiation by interacting with
nuclear steroid receptors. There is an
ongoing scientific debate concerning
the potential threat of environmental
estrogenic pollutants to animal and
human health. So far, the focus has
been on endocrinological system and
reproductive organs, but
environmental estrogens have far more
widespread actions like as bone
system. Nevertheless, the literatures
about the action of these substances on
the bone system are rare. In our study,
we found that environmental estrogens
were capable of increasing the
proliferation in primary culture of rat
osteoblast-like cells and human
osteoblast-like cell line (MG-63),
which could be blocked by estrogen
antagonist (ICI182,780). Moreover,
these environment estrogens could
also trigger the phosphorylation of
ERK1/2 and could induce the protein
expression of cyclooxygenase-2
(COX-2) in osteoblasts. MAP kinase
inhibitor PD98059 was capable of
inhibiting the expression of COX-2
and cell proliferation induced by these
environmental estrogens. These results
indicate that the MAP kinase pathway
may be involved in environment
estrogens-induced COX-2 expression
and cell proliferation in osteoblasts
Subjects
environmental estrogens osteoblasts
cell proliferation
cyclooxygenase-2
SDGs
Publisher
臺北市：國立臺灣大學醫學院檢驗醫學科
Type
journal article
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