Preparation and clinical application of immuno-magnetic latex
Date Issued
2002-07-31
Date
2002-07-31
Author(s)
DOI
902314B002300
Abstract
Peripheral blood stem cell
transplantation (PBSCT) is widely used in
this decade for cancer treatment. Allogeneic
setting can be used for various blood diseases
and autologous setting may be benefit for
solid tumors as a rescue after high-dose
chemotherapy to accelerate the bone marrow
recovery and prevent the infections or
bleeding complications. But, possibility of
tumor cells contamination is a pitfall of
autologous PBSCT while increased incidence
of graft vs. host disease may decrease the
successful rate in allogeneic PBSCT. So,
CD34+ cells selection will solve the above
problems. The commonly used and
commercially available method of CD34+
cells selection is magnetic cells sorting. But
the beads with anti-CD34+ monoclonal
antibodies are rather expensive. It needs
200,000 NT per procedure. So, We try to
bind anti-CD34 to our homemade magnetic
beads using –COOH bounding in order to do
the same purging procedure. We do believe
the homemade price will be rather cheap and
can be afforded by patients themselves. We
hope this technique will work and it can be
expanded to other negative selection such as
anti-CD34 or anti-CD8 to remove CD4+ or
CD8+ cells in order to treat some
antoimmune diseases.
Subjects
CD34+ cells
magnetic cells sorting
magnetic beads
SDGs
Publisher
臺北市：國立臺灣大學醫學院檢驗醫學科
Type
journal article
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