|Title:||Dissemination of High-Level Penicillin-, Extended-Spectrum Cephalosporin- , and Erythromycin-Resistant Streptococcus Pneumoniae Clones in Taiwan||Authors:||HSUEH, PO-REN
|Keywords:||FIELD GEL-ELECTROPHORESIS;BINDING PROTEIN GENES;ANTIMICROBIAL RESISTANCE;MULTIRESISTANT CLONE;HORIZONTAL TRANSFER||Issue Date:||1999||Journal Volume:||v.37||Journal Issue:||n.1||Start page/Pages:||221-224||Source:||JOURNAL OF CLINICAL MICROBIOLOGY||Abstract:||
Sixty-seven clinical isolates of Streptococcus pneumoniae ( 40 of serotype 23F, 19 of serotype 19F, and 8 of serotype 6B ) with decreased susceptibilities to penicillin and erythromycin were characterized by antimicrobial susceptibility patterns; DNA restriction endonuclease cleavage profiles of the penicillin-binding protein genes pbp1a, pbp2b, and pbp2x; random amplified polymorphic DNA ( RAPD) patterns generated by arbitrarily primed PCR; and chromosomal macrorestriction profiles based on pulsed-field gel electrophoresis. A total of 22 clones (identical or closely related pulsotypes and identical RAPD patterns) were identified; 14 clones of 23F, 6 of 19F, and 2 of 6B. Three 23F clones (26 isolates) and one 19F clone (9 isolates) expressed high-level resistance to penicillin, cefotaxime, and erythromycin (MICs greater than or equal to 256 mu g/ml) . These data strongly suggest that multiple high-level penicillin-, extended-spectrum cephalosporin-, and macrolide -resistant clones of S. pneumoniae have been disseminated in Taiwan.
|Appears in Collections:||醫學系|
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