https://scholars.lib.ntu.edu.tw/handle/123456789/202434
標題: | Involvement of Platelet Glycoprotein Ib in Platelet Microparticle Mediated Neutrophil Activation | 作者: | LO, SHYH-CHYI HUANG, TUR-FU LIN, DONG- TSAMN PENG, HUI-CHIN |
關鍵字: | glycoprotein Ib antagonist;leukocyte;microparticle;platelet | 公開日期: | 2006 | 卷: | v.13 | 期: | n.6 | 起(迄)頁: | 787-796 | 來源出版物: | JOURNAL OF BIOMEDICAL SCIENCE | 摘要: | Platelet microparticles (MPs) are membrane vesicles shed by platelets after activation, and carry antigens characteristic of intact platelets, such as glycoprotein (GP ) IIb/IIIa, GPIb and P-selectin. Elevated platelet MPs have been observed in many disorders in which platelet activation is documented. Recently, platelet GPIb has been implicated in the mediation of platelet-leukocyte interaction via binding to its ligand Mac-1 on leukocyte. The role of GPIb for mediating adhesion-activation interactions between platelet MPs and leukocytes has not been clarified. In this study we investigate the role of GPIb in the interplay between platelet MPs and neutrophils. Platelet MPs were obtained from collagen- stimulated platelet-rich plasma (PRP) . In a study model of neutrophil aggregation, platelet MPs can serve a bridge to support neutrophil aggregation under venous level shear stress, suggesting that platelet MPs may enhance leukocyte aggregation, which would bear clinical relevance in diseases where the platelet MPs are elevated. The level of aggregation can be reduced by GPIb blocking antibodies, AP1 and SZ2, but not by anti-CD18 mAb. The GPIb blocking antibodies also decreased platelet MP-mediated neutrophil activation, including beta2 integrin expression, adherence- dependent superoxide release and platelet MP- mediated neutrophil adherence to immobilized fibrinogen. Our data provide the evidence for the involvement of GPIb-Mac-1 interaction in the cross-talk between platelet MPs and neutrophils. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94642 |
顯示於: | 醫學系 |
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