Resveratrol在減輕大花鼠腦梗塞及再灌注性傷害中抑制嗜中性白血球黏著之發炎現象的影響

Abstract

Resveratrol, a natural phytoalexin (3,5,4’-trihydroxystilbene), is present in the seeds and skin of grapes and constitutes one of the major components of red wine. In previously study, we found that resveratrol is a potent neuroprotective agent on rats subjected to focal cerebral ischemia. The present study was undertaken to evaluate the lactate dehydrogenase (LDH) activity in plasma and to study the neutrophil infiltration in the brain after right middle cerebral artery was occluded for 1 hr and reperfusion for 24 hr in anesthetized Long-Evans rats. Compared with control groups, it revealed that pretreatment or treatment resveratrol groups, the LDH levels was decreased in plasma. In addition, compared with control groups, the change in cerebral myeloperoxidase (MPO) activity, a biochemical marker of neutrophil infiltration, which was considerably increased following focal cerebral ischemia and reduced by resveratrol pretreatment and treatment groups. We further examine whether on neutrophil resveratrol could prevent phorbol-12-myristae-13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP) induced reactive oxygen species (ROS) production. Results demonstrated that PMA and fMLP induced rapid accumulation of H2O2 in neutrophils within 30 minutes. Resveratrol decreased the accumulation of the oxygen radical metabolites. These data suggest that resveratrol is a potent neuroprotective agent in focal cerebral ischemia. Its beneficial effects may be part related to its anti-inflammatory.