Cytokine Release from Osteoblasts in Response to Different Intensities of Pulsed Electromagnetic Field Stimulation
Resource
ELECTROMAGNETIC BIOLOGY AND MEDICINE v.26 n.3 pp.153-165
Journal
ELECTROMAGNETIC BIOLOGY AND MEDICINE
Journal Volume
v.26
Journal Issue
n.3
Pages
153-165
Date Issued
2007
Date
2007
Author(s)
Li, Jimmy Kuan-Jung
Lin, James Cheng-An
Liu, Hwa Chang
Chang, Walter Hong-Shong
Abstract
We use an in-vitro osteoblast cell culture model to investigate the effects of low-frequency (7.5Hz) pulsed electromagnetic field ( PEMF) stimulation on osteoblast population, cytokines (prostaglandin E-2 (PGE(2) ), transforming growth factor beta 1(TGF beta 1), and alkaline phosphatase ( ALP) activity to find the optimal intensity of PEMF for osteoblast growth. The results demonstrate that PEMF can stimulate osteoblast growth, release of TGF beta 1, and, in addition, an increase of ALP activity. The synthesis and release of PGE(2) in the culture medium are reduced with increasing numbers of cells. Higher intensity does not necessarily mean increased osteoblast growth, and the most efficient intensity is about 2mV /cm in this case. Although the lower intensities of the PEMF are yet to be determined, the results of this study can shed light on the mechanisms of PEMF stimulation on non union fracture therapy and osteoporosis prevention in the future.
Subjects
ALP
intensity
osteoblasts
PEMF
PGE(2)
TGF beta 1