https://scholars.lib.ntu.edu.tw/handle/123456789/204920
標題: | Tgf-Beta1 Immobilized Tri-Co-Polymer for Articular Cartilage Tissue Engineering | 作者: | CHOU, CHENG-HUNG CHENG, WINSTON T. K. LIN, CHIEN-CHENG CHANG, CHIH-HUNG TSAI, CHIEN-CHEN LIN, FENG-HUEI |
關鍵字: | TGF-beta 1;gelatin;chondroitin-6-sulfate;hyaluronic acid;carbodiimide;articular cartilage | 公開日期: | 2006 | 卷: | v. | 期: | n.2 | 起(迄)頁: | 338-348 | 來源出版物: | Journal of Biomedical Materials Research - Part B Applied Biomaterials | 摘要: | Tri-co-polymer with composition of gelatin, hyaluronic acid and chondroitin-6-sulfate has been used to mimic the cartilage extracellular matrix as scaffold for cartilage tissue engineering. In this study, we try to immobilize TGF- beta1 onto the surface of the tri-co-polymer sponge to suppress the undesired differentiation during the cartilage growth in vitro. The scaffold was synthesized with a pore size in a range of 300-500 microm. TGF-beta1 was immobilized on the surface of the tri-co-polymer scaffold with 1-ethyl- 3-(3-dimethylaminopropyl) carbodiimide (EDC) as a crosslinking agent. Tri-co-polymer scaffolds with and without TGF-beta1 were seeded with porcine chondrocytes and cultured in a spinner flask for 2, 4, and 6 weeks. The chondrocytes were characterized by the methods of immunohistochemical staining with anti-type II collagen and anti-S-100 protein monoclonal antibody, and RT-PCR. After culturing for 4 weeks, chondrocytes showed positive in S-100 protein, Alcian blue, and type II collagen for the scaffold with TGF-beta1 immobilization. There is no observed type I and type X collagen expression in the scaffolds from the observation of RT-PCR. In addition, the scaffold without TGF -beta1 immobilization, type X collagen, can be detected after cultured for 2 weeks. Type I collagen was progressively expressed after 4 weeks. These results can conclude that TGF-beta1 immobilized scaffold can suppress chondrocytes toward prehypertrophic chondrocytes and osteolineage cells. The tri-co-polymer sponge with TGF-beta1 immobilization should have a great potential in cartilage tissue engineering in the future. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94107 |
顯示於: | 醫學系 |
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