https://scholars.lib.ntu.edu.tw/handle/123456789/204927
標題: | Fabrication and Release Behavior of a Novel Freeze-Gelled Chitosan/Gamma- Pga Scaffold as a Carrier for Rhbmp-2 | 作者: | HSIEH, HSYUE-JEN LIU, HWA-CHANG WANG, DA-MING HOU, LEIN-TUAN |
關鍵字: | chitosan;gamma-PGA;rhBMP-2;controlled release | 公開日期: | 2006 | 卷: | v.22 | 期: | n.7 | 起(迄)頁: | 622-629 | 來源出版物: | Dental Materials | 摘要: | Objective: The aim of this study was to fabricate a novel composite porous scaffold by blending chitosan and gamma(.) poly(glutamic acid) ( gamma-PGA) for the sustained delivery of rhBMP-2. Methods: Chitosan and gamma-PGA were blended to fabricate a novel porous scaffold by the freeze- gelation method. For comparison, scaffolds made of freeze-dried chitosan, freeze-dried PLLA, and freeze-gelled chitosan were also prepared. The scaffolds were loaded with rhBMP-2, and then the controlled release of rhBMP-2 from the scaffolds was assessed by ELISA. Results: The freeze- gelled chitosan/ gamma-PGA scaffold (M-o = 318.29 ng, k = 0.32 d (1)) gave the most satisfactory release curve, followed by the freeze- gelled chitosan (M-o = 392.76 ng, k = 0.59 d (1)), freeze- dried chitosan (M-o = 229.21 ng k = 2 28 d (1)), and freeze- dried PLLA (M-o = 8.4 ng, k = 482. 54 d (1)) scaffolds. In the stability test, p-dioxane (the solvent for PLLA) seriously deteriorated rhBMP-2, whereas acetic acid (the solvent for chitosan) did not. Significance: A novel chitosan/gamma-PGA composite scaffold for the controlled release of rhBMP-2 was established, with an enhanced release amount and sustained release behavior. This scaffold has many potential applications in bone regenerative therapies.( c) 2005 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94116 |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。