https://scholars.lib.ntu.edu.tw/handle/123456789/204958
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 骨科 | en |
dc.contributor.author | CHIANG, HUEI-SHIEN | en |
dc.contributor.author | YANG, RONG-SEN | en |
dc.contributor.author | HUANG, TUR-FU | en |
dc.creator | 江慧嫻;楊榮森;黃德富 | zh-tw |
dc.creator | CHIANG, HUEI-SHIEN;YANG, RONG-SEN;HUANG, TUR-FU | en |
dc.date | 1995 | en |
dc.date.accessioned | 2009-01-19T01:52:21Z | - |
dc.date.accessioned | 2018-07-13T13:43:03Z | - |
dc.date.available | 2009-01-19T01:52:21Z | - |
dc.date.available | 2018-07-13T13:43:03Z | - |
dc.date.issued | 1995 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/99101 | - |
dc.description.abstract | Saos-2 cells, derived from a primary human osteosarcoma, caused dose-dependent platelet aggregation in heparinized human platelet-rich plasma. Saos-2 tumour cell-induced platelet aggregation (TCIPA) was completely inhibited by hirudin but unaffected by apyrase. The cells suspension shortened the plasma recalcification times of normal, factor VIII-deficient and factor IX- deficient human plasmas in a dose- dependent manner. However , the cell suspension did not affect the recalcification time of factor VII-deficient plasma. Moreover, a monoclonal antibody(MAb) against human tissue factor completely abolished TCIPA. Flow cytometric analysis using anti- integrin MAbs as the primary binding ligands demonstrated that the integrin receptors αvβ3, α5β1 and α6β1 were present on the surface of Saos -2 cells, which might mediate tumour cell adhesion to extracellular matrix. Rhodostomin, an Arg- Gly-Asp(RGD)-containing snake venom peptide which antagonises the binding of fibrinogen to platelet membrane glycoprotein IIb/IIIa, prevented Saos-2 TCIPA as well as tumour cell adhesion to vitronectin, fibronectin and collagen type I. Likewise, the synthetic peptide Gly-Arg-Gly-Asp-Ser (GRGDS) showed a similar effect. On a molar basis, rhodostomin was about 18, 000 and 1,000 times, respectively, more potent than GRGDS in inhibiting TCIPA and tumour cell adhesion.#2046# | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | BRITISH JOURNAL OF CANCER v.71 n.2 pp.265-270 | en |
dc.relation.ispartof | BRITISH JOURNAL OF CANCER | - |
dc.title | The Arg-Gly-Asp-containing peptide, rhodostomin, inhibits in vitro cell adhesion to extracellular matrices and platelet aggregation caused by Saos -2 human osteosarcoma cells | en |
dc.relation.pages | 265-270 | - |
dc.relation.journalvolume | v.71 | - |
dc.relation.journalissue | n.2 | - |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。