Effect of Different Solvents and Crosslinkers on Cytocompatibility of Type Ii Collagen Scaffolds for Chondrocyte Seeding
Resource
ARTIFICIAL ORGANS v.26 n.1 pp.18-26
Journal
ARTIFICIAL ORGANS
Journal Volume
v.26
Journal Issue
n.1
Pages
18-26
Date Issued
2002
Date
2002
Author(s)
TSAI, CHING-LIN
Abstract
Type II collagen extracted from porcine costal cartilage was evaluated as scaffolds for cartilage tissue engineering. Chemical crosslinkers were employed to improve the mechanical properties and the resistance toward degradation. Films and porous scaffolds were prepared from collagen solutions dissolved in 3% acetic acid (designated A) or in deionized water (designated W) and crosslinked by an epoxy ( designated E) or by a carbodiimide (designated C). Immortalized rat chondrocytes and rabbit chondrocytes were used to assess cytocompatibility of crosslinked collagen matrices. Cell adhesion rate onto the films made by different preparations ranked in the order of WE greater than or equal to WC > AC greater than or equal to AE. Cell proliferation ranked in the order of AC > WC > AE WE. Cells maintained round morphology only on AC and WC Films. In 3-D seeding, AC scaffolds also were found to be the most cytocompatible . WC scaffolds, however, had better dimensional stability. It was concluded that Type II collagen scaffolds, when prepared by using deionized water as the solvent and carbodiimide as the crosslinker, could promote chondrocyte growth and matrix production.
Subjects
scaffolds chondrocytes
Type II collagen
crosslinkers
extracellular matrix
ARTICULAR CHONDROCYTES