https://scholars.lib.ntu.edu.tw/handle/123456789/288896
標題: | Suppression of lipopolysaccharide-induced nuclear factor-κB activity by theaflavin-3,3'-digallate from black tea and other polyphenols through down-regulation of IκB kinase activity in macrophages | 作者: | MIN-HSIUNG PAN Lin-Shiau, S.-Y. Ho, C.-T. Lin, J.-H. Lin, J.-K. |
關鍵字: | IκB kinase; Inducible nitric oxide synthase; Inhibitor κB; Macrophage; Nuclear factor-κB; RAW 264.7; Theaflavin-3,3'-digallate | 公開日期: | 2000 | 卷: | 59 | 期: | 4 | 起(迄)頁: | 357-367 | 來源出版物: | Biochemical Pharmacology | 摘要: | We investigated the inhibition of IκB kinase (IKK) activity in lipopolysaccharide (LPS)-activated murine macrophages (RAW 264.7 cell line) by various polyphenols including (-)-epigallocatechin-3-gallate, theaflavin, a mixture of theaflavin-3 gallate and theaflavin-3'-gallate, theaflavin-3,3'-digallate (TF-3), pyrocyanidin B-3, casuarinin, geraniin, and penta-O-galloyl-β-D-glucose (5GG). TF-3 inhibited IKK activity in activated macrophages more strongly than did the other polyphenols. TF-3 strongly inhibited both IKK1 and IKK2 activity and prevented the degradation of IκBα and IκBβ in activated macrophage cells. The results suggested that the inhibition of IKK activity by TF-3 could occur by a direct effect on IKKs or on upstream events in the signal transduction pathway. Furthermore, geraniin, 5GG, and TF-3 all blocked phosphorylation of IκB from the cytosolic fraction, inhibited nuclear factor-κB (NFκB) activity, and inhibited increases in inducible nitric oxide synthase levels in activated macrophages. These results suggest that TF-3 may exert its anti-inflammatory and cancer chemopreventive actions by suppressing the activation of NFκB through inhibition of IKK activity. Copyright (C) 2000 Elsevier Science Inc. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0033976073&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/288896 |
DOI: | 10.1016/S0006-2952(99)00335-4 | SDG/關鍵字: | casuarinin; epigallocatechin gallate; geraniin; glucose pentagallate; immunoglobulin enhancer binding protein; lipopolysaccharide; nitric oxide synthase; phosphotransferase; polyphenol; procyanidin; theaflavin; theaflavine 3 gallate; theaflavine 3' gallate; theaflavine digallate; thearubigin; unclassified drug; animal cell; article; controlled study; cytotoxicity; dose response; electrophoretic mobility; enzyme activity; enzyme induction; macrophage; macrophage activation; nonhuman; priority journal; Animals; Antioxidants; Biflavonoids; Catechin; Cells, Cultured; Down-Regulation; Enzyme Inhibitors; Flavonoids; Gallic Acid; Gene Expression; Glucosides; Hydrolyzable Tannins; I-kappa B Kinase; Lipopolysaccharides; Macrophages; Mice; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Phenols; Phosphorylation; Polymers; Protein-Serine-Threonine Kinases; Tannins; Tea |
顯示於: | 食品科技研究所 |
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