Electrophoretic separation of DNA in the presence of electroosmotic flow
Journal
Reviews in Analytical Chemistry
Journal Volume
19
Journal Issue
1
Pages
45-74
Date Issued
2000
Author(s)
Abstract
The common methods and theories of DNA separation in capillary electrophoresis (CE) using polymer solutions are briefly described. In this article, we focus on reviewing our recently developed techniques for DNA separation in the presence of EOF. These techniques are different from conventional DNA separation methods with respect to separation procedure, detection end, separation order, and factors affecting resolutions. First, DNA separation was performed using capillaries without filling with polymer solutions prior to analysis. Second, DNA fragments migrate towards the cathode by the EOF. Third, larger DNA fragments are detected earlier than smaller ones. Fourth, resolution between a pair of DNA fragments is dependent not only on properties of polymer solutions, characteristics of DNA fragments, buffer additives, and electric Field strengths, but also on the EOF. Last, gradient techniques such as using different concentrations of polymer solutions, intercalated dyes, and additives are easily generated. The results suggest that possible applications of this technique include DNA sequencing, analysis of PCR products, restriction fragments, and other biopolymers.
Type
journal article