Preferential loss of a polymorphic RIZ allele in human hepatocellular carcinoma
Journal
British Journal of Cancer
Journal Volume
84
Journal Issue
6
Pages
743-747
Date Issued
2001
Author(s)
Abstract
The RIZ (PRDM2) locus commonly undergoes loss of heterozygosity (LOH) and maps within the minimal deleted region on 1p36 in hepatocellular carcinoma (HCC). Although peptide-altering mutations of RIZ are rare in HCC, the RIZ1 product is commonly lost in HCC and has tumour suppressive activities. Here, we analysed RIZ gene mutations and LOH in HCC, breast cancer, familial melanoma, colon cancer, and stomach cancer. We found 7 polymorphisms but no mutations. By analysing the Pro704-deletion polymorphism, we detected LOH of RIZ in 31 of 79 (39%) informative HCC cases, 11 of 47 (23%) colon cancer cases, 8 of 43 (19%) breast cancer cases, 8 of 66 (12%) stomach cancer cases. Importantly, loss of the Pro704+ allele was found in 74% of the 31 LOH positive HCC cases (P < 0.01), indicating a preferential loss and hence a stronger tumour suppressor role for this allele compared to the P704- allele. In addition, the Pro704+ allele was found to be more common in Asians (0.61) than Caucasians (0.42) (P = 0.0000), suggesting an interesting link between gene polymorphisms and potential differences in tumour incidence between racial groups. ? 2001 Cancer Research Campaign.
Subjects
Hepatoma; Polymorphisms; RIZ (PRDM2)
SDGs
Other Subjects
Taq polymerase; allele; article; Asian; breast cancer; cancer incidence; cancer inhibition; Caucasian; colon cancer; controlled study; deletion mutant; familial disease; gene locus; gene mapping; gene mutation; genetic polymorphism; heterozygosity loss; human; human tissue; liver cell carcinoma; major clinical study; melanoma; priority journal; race difference; single strand conformation polymorphism; stomach cancer; Alleles; Base Sequence; Breast Neoplasms; Carcinoma, Hepatocellular; Chromosome Deletion; DNA Primers; DNA-Binding Proteins; Humans; Liver Neoplasms; Melanoma; Mutation; Nuclear Proteins; Polymorphism, Genetic; Transcription Factors
Type
journal article