Species Identification of Anguillid Eels by Polymerase Chain Reaction / Restriction Fragment Length Polymorphism Analysis of the Gonadotropin II- βSubunit Gene
Journal
Acta Zoologica Taiwanica
Journal Volume
12
Journal Issue
2
Pages
41 - 49
Date Issued
2001
Author(s)
Abstract
Gonadotropin II beta-subunit gene (GTH II- β DNA) fragments of nine eel species of the genus Anguilla were amplified with specific oligonucleotides by polymerase chain reaction (PCR), and a single product was obtained from each species. In subsequent restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified GTH II- β DNA, the restriction endonucleases Hpa II, Sph I, and Nsi 1 gave restriction patterns that differed among three species, e.g., Anguilla japonica, A. anguilla and A. bicolor bicolor, and from the other six species of anguillid eels,i.e., A. rostrata, A. australis australis, A. reinhardti, A. celebesensis, A. marmorata, and A. bicolor pacifica. Our results suggest that PCR/RFLP analysis provides a rapid and convenient method to discriminate eel species.
SDGs
Type
journal article