https://scholars.lib.ntu.edu.tw/handle/123456789/292213
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | MEI-RU CHEN | en_US |
dc.contributor.author | Liu, Mei-Ying | en_US |
dc.contributor.author | Hsu, Shih-Mei | en_US |
dc.contributor.author | Fong, Chin-Chu | en_US |
dc.contributor.author | Chen, Chien-Jen | en_US |
dc.contributor.author | Chen, I-How | en_US |
dc.contributor.author | Hsu, Mow-Ming | en_US |
dc.contributor.author | Yang, Czau-Siung | en_US |
dc.contributor.author | Chen, Jen-Yang | en_US |
dc.creator | Chen, M.-R.;Liu, M.-Y.;Hsu, S.-M;Fong, C.-C.;Chen, C.-J.;Chen, I.H.;Hsu, M.-M.;Yang, C.-S;Chen, J.-Y | - |
dc.date.accessioned | 2018-09-10T03:44:10Z | - |
dc.date.available | 2018-09-10T03:44:10Z | - |
dc.date.issued | 2001 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-0035085293&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/292213 | - |
dc.description.abstract | EBV serological tests have been used for many years as accessory diagnostic predictors of nasopharyngeal carcinoma (NPC). To increase the sensitivity and specificity of the NPC detection rate, a novel enzyme-linked immunosorbent assay (ELISA) was established using a bacterially-expressed GST-EBNA-1 protein, containing the EBNA-1 sequence cloned from an NPC patient. Serum samples were collected from age- and gender-matched patients with NPC, community control subjects and hospital control patients and tested using this ELISA. The positivity rates were 78.7% (247/314) in NPC, 11.5% (28/244) in hospital controls and 3.8% (10/263) in the community control group. These serum samples were also tested for IgA anti-VCA antibodies and their ability to neutralize EBV DNase and the sensitivities of the anti-VCA antibody and DNase-neutralization tests also were analyzed. The optimum combination is VCA plus EBNA-1, which can identify 92.5% (287/310) of NPC patients, and shows a specificity of 92.7% (242/261) for normal individuals. ? 2001 Wiley-Liss, Inc. | en_US |
dc.language | en | en |
dc.relation.ispartof | Journal of Medical Virology | en_US |
dc.source | AH | - |
dc.subject | Antibodies; EBNA-1; NPC | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | cell nucleus antigen; deoxyribonuclease; EBNA 1 protein; Epstein Barr virus antigen; glutathione transferase; unclassified drug; virus capsid antigen; article; cancer diagnosis; cancer screening; controlled study; diagnostic accuracy; diagnostic procedure; enzyme linked immunosorbent assay; Epstein Barr virus; human; nasopharynx carcinoma; screening test; serodiagnosis; Antibodies, Viral; Antigens, Viral; Biopsy; Capsid Proteins; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Epitopes; Epstein-Barr Virus Nuclear Antigens; Escherichia coli; Humans; Immunoglobulin A; Matched-Pair Analysis; Nasopharyngeal Neoplasms; Neutralization Tests; Plasmids; Recombinant Fusion Proteins; Sensitivity and Specificity; Serologic Tests; Taiwan | - |
dc.title | Use of bacterially expressed EBNA-1 protein cloned from a nasopharyngeal carcinoma (NPC) biopsy as a screening test for NPC patients | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/jmv.1017 | - |
dc.relation.pages | 51-57 | en_US |
dc.relation.journalvolume | 64 | en_US |
dc.relation.journalissue | 1 | en_US |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
crisitem.author.dept | Microbiology | - |
crisitem.author.orcid | 0000-0002-7007-8036 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。