Evaluation of a polymerase chain reaction-based system for detecting Salmonella species from pork carcass sponge samples
Journal
Journal of Food Science
Journal Volume
68
Journal Issue
3
Pages
992-995
Date Issued
2003
Author(s)
Wu, C.-C.
Liu, C.-I.
Tsai, C.-E.
Yeh, K.-S.
Abstract
Pork carcass sponge samples (n = 230) collected from 10 Taiwanese slaughter plants were screened for Salmonella using 2 methods: BAX® for Screening/Salmonella, a polymerase chain reaction-based detection system and a culture method using SM-ID agar as the selective plating medium. The BAX method identified 14 samples as positive for Salmonella. The 8 samples identified as Salmonella-positive by the SM-ID method were also BAX-positive. Inoculation studies showed BAX detected Salmonella in samples having initial 1.4 × 101 cfu/mL Salmonella inoculum prior to the enrichment process in the presence of 3.0 × 106 cfu/mL of non-Salmonella florae. BAX provides a rapid screening alternative to the culture method for Salmonella detection.
Type
journal article