Starch-accumulating sweet potato callus tissue devoid of β-amylase but with two starch phosphorylase isozymes
Journal
Bioscience, Biotechnology and Biochemistry
Journal Volume
57
Journal Issue
8
Pages
1311-1315
Date Issued
1993
Author(s)
Abstract
By controlling the concentrations of kinetin, auxin, and sucrose in the Murashige-Skoog medium, starch contents in callus culture induced from sweet potato tissues could be manipulated. Activity staining and Western analysis on PAGE plates and activity assays made on starch phosphorylase in the presence and absence of mercuric ions showed that β-amylase is absent in callus cultures regardless of whether their starch content is high or low. This would imply that β-amylase induction in sweet potato calli is not linked to the metabolic control through which the expression of storage function is associated, as proposed by Nakamura et al. [Plant Physiol., 96, 902 (1991)] for sweet potato leaf-petiole cuttings. Analyses of starch phosphorylase in crude extracts suggested the presence of a new starch phosphorylase in tuberous root and callus tissue. This phosphorylase is immunologically different from the tuberous root and leaf enzymes that we studied previously. © 1993, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.
SDGs
Type
journal article