https://scholars.lib.ntu.edu.tw/handle/123456789/307763
標題: | Comparative Genomics of Rickettsia prowazekii Madrid E and Breinl Strains | 作者: | Ge, H. ERIC YAO-YU CHUANG Zhao, S. Tong M MONG-HSUN TSAI Temenak J.J Richards A.L Ching W.-M. ERIC YAO-YU CHUANG |
公開日期: | 一月-2004 | 卷: | 186 | 期: | 2 | 起(迄)頁: | 556-565 | 來源出版物: | Journal of Bacteriology | 摘要: | Rickettsia prowazekii, the causative agent of epidemic typhus, has been responsible for millions of human deaths. Madrid E is an attenuated strain of R. prowazekii, while Breinl is a virulent strain. The genomic DNA sequence of Madrid E has recently been published. To study the genomic variations between Madrid E (reference) and Breinl (test) DNAs, cohybridization experiments were performed on a DNA microarray containing all 834 protein-coding genes of Madrid E. Of the 834 genes assessed, 24 genes showed 1.5- to 2.0-fold increases in hybridization signals in Breinl DNA compared to Madrid E DNA, indicating the presence of genomic variations in ∼3% of the total genes. Eighteen of these 24 genes are predicted to be involved in different functions. Southern blot analysis of five genes, virB4, ftsK, rfbE, lpxA, and rpoH, suggested the presence of an additional paralog(s) in Breinl, which might be related to the observed increase in hybridization signals. Studies by real-time reverse transcription-PCR revealed an increase in expression of the above-mentioned five genes and five other genes. In addition to the elevated hybridization signals of 24 genes observed in the Breinl strain, one gene (rp084) showed only 1/10 the hybridization signal of Madrid E. Further analysis of this gene by PCR and sequencing revealed a large deletion flanking the whole rp084 gene and part of the rp083 gene in the virulent Breinl strain. The results of this first rickettsial DNA microarray may provide some important information for the elucidation of pathogenic mechanisms of R. prowazekii. |
URI: | http://www.ncbi.nlm.nih.gov/pubmed/14702324 http://scholars.lib.ntu.edu.tw/handle/123456789/307763 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0347915667&doi=10.1128%2fJB.186.2.556-565.2004&partnerID=40&md5=fa599147d1f1545ad5fb4104050afa76 |
ISSN: | 00219193 | DOI: | 10.1128/JB.186.2.556-565.2004 | SDG/關鍵字: | bacterial DNA; genomic DNA; article; bacterial gene; bacterial genetics; bacterial strain; bacterial virulence; cause of death; comparative genomic hybridization; DNA determination; DNA hybridization; DNA microarray; DNA sequence; ftsk gene; gene deletion; gene sequence; genetic analysis; lpxa gene; nonhuman; pathogenesis; polymerase chain reaction; priority journal; rfbe gene; Ricketsia prowazekii breinl; Ricketsia prowazekii Madrid E; Rickettsia prowazekii; rp083 gene; rp084 gene; rpoh gene; Southern blotting; typhus; virb4 gene; Blotting, Southern; Carbohydrate Epimerases; DNA, Bacterial; Genome, Bacterial; Heat-Shock Proteins; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; Rickettsia prowazekii; Sigma Factor; Transaminases; Transcription Factors; Virulence; Bacteria (microorganisms); Prokaryota; Rickettsia; Rickettsia prowazekii; Rickettsia prowazekii str. Madrid E; Rickettsiales |
顯示於: | 生醫電子與資訊學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。