https://scholars.lib.ntu.edu.tw/handle/123456789/309387
標題: | Genetic variation of viral protein 1 genes of field strains of waterfowl parvoviruses and their attenuated derivatives | 作者: | HSIANG-JUNG TSAI Tseng, C.-H. Chang, P.-C. Mei, K. Wang, S.-C. |
關鍵字: | Goose parvovirus; Muscovy duck parvovirus; Nucleotide sequences; Viral protein 1 (VP1); Waterfowl parvovirus | 公開日期: | 2004 | 卷: | 48 | 期: | 3 | 起(迄)頁: | 512-521 | 來源出版物: | Avian Diseases | 摘要: | To understand the genetic variations between the field strains of waterfowl parvoviruses and their attenuated derivatives, we analyzed the complete nucleotide sequences of the viral protein 1 (VP1) genes of nine field strains and two vaccine strains of waterfowl parvoviruses. Sequence comparison of the VP1 proteins showed that these viruses could be divided into goose parvovirus (GPV) related and Muscovy duck parvovirus (MDPV) related groups. The amino acid difference between GPV- and MDPV-related groups ranged from 13.1% to 15.8%, and the most variable region resided in the N terminus of VP2. The vaccine strains of GPV and MDPV exhibited only 1.2% and 0.3% difference in amino acid when compared with their parental field strains, and most of these differences resided in residues 497-575 of VP1, suggesting that these residues might be important for the attenuation of GPV and MDPV. When the GPV strains isolated in 1982 (the strain 82-0308) and in 2001 (the strain 01-1001) were compared, only 0.3% difference in amino acid was found, while MDPV strains isolated in 1990 (the strain 90-0219) and 1997 (the strain 97-0104) showed only 0.4% difference in amino acid. The result indicates that the genome of waterfowl parvovirus had remained highly stable in the field. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-13744251251&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/309387 |
DOI: | 10.1637/7172 | SDG/關鍵字: | Anas sp.; Anatidae; Anser sp.; Cairina moschata; Duck parvovirus; Goose parvovirus; Muscovy duck parvovirus; Parvovirus; primer DNA; virus protein; amino acid sequence; animal; article; cluster analysis; comparative study; DNA sequence; duck; genetic variability; genetics; goose; molecular genetics; nucleotide sequence; Parvovirus; phylogeny; sequence alignment; sequence homology; species difference; Taiwan; virology; Amino Acid Sequence; Animals; Base Sequence; Cluster Analysis; DNA Primers; Ducks; Geese; Molecular Sequence Data; Parvovirus; Phylogeny; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology; Species Specificity; Taiwan; Variation (Genetics); Viral Proteins |
顯示於: | 獸醫學系 |
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